GTP-responsive tubulin vesicle with a photoreactive molecular glue for DDS carrier
-
1
Riken, Japan
-
2
The University of Tokyo, Department of Chemistry and Biotechnology, Japan
Microtubule is a representative biomolecular machine composed of tubulin dimers, and reveals the machinery action derived from the conformational change of tubulin units responsive to guanosine triphosphate (GTP), in which depolymerization and reconfiguration of microtubule take place repeatedly. We found that microtubule can be reconfigured to tubulin sheets by the manipulation of self-assembly of tubulin units by an addition of GTP and non-hydrolyzable GTP analog under the depolymerization process of microtubule (Figure 1). Then, it was serendipitously unveiled that tubulin sheets were folded into hollow sphere by the action of a photoreactive molecular glue that can glue proteins together stepwisely[1] (Figure 1). Upon mixing with GTP, tubulin hollow sphere reveals machinery action synchronized with the tubulin units. As a result, tubulin hollow sphere collapses and releases included drug molecules. Noteworthy, this GTP-responsive tubulin vesicle serves as a drug delivery system (DDS) carrier for intracellular delivery to cancer cells which produce highly concentrated GTP.[2]
References:
[1] N. Uchida, et al. J. Am. Chem. Soc. 2013, 135, 4684.
[2] N. Uchida, et al. to be submitted.
Keywords:
self-assembly,
Drug delivery,
protein,
Capsule
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Protein-based biomaterials
Citation:
Uchida
N,
Okuro
K and
Aida
T
(2016). GTP-responsive tubulin vesicle with a photoreactive molecular glue for DDS carrier.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01385
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Noriyuki Uchida, Riken, Wako, Japan, noriyuki.uchida@riken.jp
Dr. Kou Okuro, The University of Tokyo, Department of Chemistry and Biotechnology, Hongo, Japan, okuro@macro.t.u-tokyo.ac.jp
Dr. Takuzo Aida, Riken, Wako, Japan, aida@macro.t.u-tokyo.ac.jp