Does a surface coating inhibit bacterial adhesion on silicone hydrogel contact lenses
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1
Johnson & Johnson Vision Care inc, Research & Development, United States
Purpose: Adhesion of bacteria to contact lens (CL) surfaces is a major contributory factor in the development of CL adverse events. This study sought to determine if surface treating silicone hydrogel (SiHy) CL offers any substantial benefits against adhesion of gram positive and negative bacteria.
Method: Two surface-treated SiHy CL (lotrafilcon B; delefilcon A) & 3 uncoated SiHy CL (comfilcon A; samfilcon A; senofilcon A) from manufacturers packaging were soaked in an artificial tear mimicking solution for 15 h under rotative agitation at 35°C. Lenses (n=15) were then rinsed and challenged with either P aeruginosa ATCC 9027 & S. aureus ATCC 25923, using an inoculum of approx. 1x106 colony forming units per milliliter (CFU/mL) for 15 h. Adherent bacteria were recovered and the bacterial supernatant was enumerated for viable microorganisms by a spiral plate technique. CFU counts were determined using an automated colony counter.
Results: The 2 surface-treated SiHy CL (lotrafilcon B and delefilcon A) exhibited average Log colony forming units (CFU) of 5.64 & 5.86 for P. aeruginosa and 3.74 & 3.83 for S. aureus respectively. The counts for the 3 uncoated SiHy CL (comfilcon A; samfilcon A; senofilcon A) were 5.86, 5.86 & 5.70 for P. aeruginosa and 3.89, 3.85 & 4.02 for S. aureus. No significant difference was determined between surface-treated and untreated materials (p=0.268). P. aeruginosa exhibited a higher propensity to adhere to the materials than S. aureus (p<0.001).
Conclusions: In vitro bacterial adhesion was not modified by applying a surface treatment to the lens materials evaluated and all materials accumulated the gram negative bacteria to a greater extent than the gram positive organism. This suggests that the surface treatments applied to lotrafilcon B and delefilcon A does not provide a protective effect from bacterial adhesion. Further evaluation of the materials post-wear is required to validate the clinical relevance of these in vitro findings.
Centre for Contact lens research at the University of Waterloo
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials for ophthalmic applications
Citation:
Mccabe
K
(2016). Does a surface coating inhibit bacterial adhesion on silicone hydrogel contact lenses.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.02416
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.