Event Abstract

Graphene oxide reduction as a result of cells activity

  • 1 Medical University of Warsaw, Department of Histology and Embryology, Poland
  • 2 Medical University of Warsaw, Centre for Preclinical Research and Technology, Poland
  • 3 Warsaw University of Technology, Faculty of Physics, Poland
  • 4 Institute of Electronic Materials Technology, Poland

Graphene oxide (GO) and reduced GO (rGO) are attractive materials with unique physicochemical properties and high potential for use in tissue engineering. These two materials have different properties, e.g. hydrophilicity, affinity to biomolecules or electronic conductibility. Several biological substances are known to reduce GO, however, little is known about the reduction of GO and changes of its properties induced by cells cultured on its surface. The switch of the properties during cell culture from e.g. hydrophilic (GO) to hydrophobic (rGO) may have a great impact on material-cell interactions. Therefore, the aim of this study was to investigate how cells and standard cell culture procedures modify GO.

GO was prepared by a modified Hummers method. GO (2 mg/ml) was cast on the surface of the 6-well plates. After drying, GO-covered wells were seeded with 2x105 of human adipose-derived mesenchymal stem cells (ADSC), cultured until sub-confluence and subjected to various manipulations including treatment with trypsin (0.1%), lysis buffer (0.1% Triton, 10mM Tris-HCl), and 10% buffered formalin. Next, GO samples were analyzed by Raman spectroscopy. The GO reduction level was measured by relative intensity ratio (ID/IG) of two main Raman peaks (D and G). Reduction of GO was also confirmed by increased quenching of fluorescence.

We demonstrate that ADSC culture on GO surface leads to significant ID/IG increase – from ~1.75 to the range of 1.85 to 2 (depending on the applied treatment), which indicates GO to rGO conversion. Fluorescence quenching confirmed the reduction of GO in a consequence of ADSC culture and manipulation.

Keywords: stem cell, electric, Nano/micro particle

Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.

Presentation Type: Poster

Topic: Biomaterials in mesenchymal and hematopoietic stem cell biology

Citation: Kalaszczynska I, Zdrojek M, Wroblewska A, Judek J, Ruminski S, Baran M, Lipinska L and Lewandowska-Szumiel M (2016). Graphene oxide reduction as a result of cells activity. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02470

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Received: 27 Mar 2016; Published Online: 30 Mar 2016.