Chitosan/Bioglass composite films by dip-coating method
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1
University of Campinas – UNICAMP, Institute of Chemistry, Brazil
Nanostructured polymer/bioglass composites have generated promising results in bone tissue engineering. The presence of the bioactive phase at the nanometer scale increases the bioactivity and improves the mechanical strength of final the material.[1] Chitosan is a polysaccharide obtained by deacetylation of chitin that exhibits biocompatibility, antibacterial activity and biodegradability properties.[2] On the other hand, bioactive glasses exhibit a high bioactivity, osteoconductivity and osteostimulative properties through a direct control over genes that regulate the induction and cell cycle progression.[2][3] In this study we report the production of films combining chitosan hydrogel (CHT) with the precursor solution (sol) of 58S bioglass (PBG), aiming at achieving a novel composite biomaterial with superior mechanical, thermal and biological properties compared to pure chitosan. The hydrogel was prepared by mixing chitosan and distilled water (2 % w/w) and, subsequently, the concentrated hydrochloric acid was added in stirring until the complete solubilization of polysaccharide. Thereafter, were prepared three CHT systems containing different amounts ofthe precursor solution (sol) of 58S bioglass (PBG): 12, 33, and 50 wt/wt% (PBG/CHT ratio). Films were prepared by dip-coating method with 5 layers of CHT/PBG. The samples were characterized for several techniques, such as Raman and Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM). In addition, in vitro tests by immersion in simulated body fluid solution (SBF) were evaluated. Figure 1 shows some representative SEM micrographs of the pure chitosan films (CHT) and the composite films (CHT/PBG). Figure 1. SEM micrographs of (a) pure CHT, (b) CHT/PBG 33%, (c) CHT/PBG 33%, and (d) CHT/PBG 50 % films. The morphology observed in Figure 1a is consistent with pure chitosan, whereas the surface of the CHT/PBG films present asperities corresponding to the presence of partially exposed bioglass particles. In addition, SEM images of the films demonstrates that the BG particles are homogeneously distributed on the films surface. Raman and FTIR spectroscopies suggest the presence of rich-silica clusters homogeneously dispersed throughout the film. These clusters are chemically interacting with chitosan. Furthermore, mechanical and thermal properties were considerably improved for CHT/PBG compared to that of pure chitosan. In vitro tests for CHT/PBG showed a high ability to induce the precipitation of apatite in SBF.
This work was carried out with the support of the CNPq and PETROBRAS.
References:
[1] S.G. Caridade, E.G. Merino, N.M. Alves, V.Z. Bermudez, A.R. Boccacini, J.F.Mano J. Of the Mechanical Behavior of Biomedical Materials 2013;20: 173-183.
[2] F. Croisier, C. Jérôme European Polymer Journal 2013,49,780-792.
[3] Q.Z. Chen, I.D. Thompson, A.R. Boccaccini. Biomaterial 2006;27:2414-2425
Keywords:
Bioactivity,
Biocompatibility,
composite,
Biodegradable material
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomaterials in constructing tissue substitutes
Citation:
Lopes
J,
Aguiar
A,
Signorelli
F,
Barbosa
G and
Bertran
C
(2016). Chitosan/Bioglass composite films by dip-coating method.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.02920
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.