Event Abstract

Toxic effects caused by a long-term exposure of Danio rerio to TiO2 nanoparticles.

  • 1 University of Catania (Italy), Department of Biological, Geological and Environmental Sciences, Italy
  • 2 Agricultural University of Athens, Greece
  • 3 CNR-IMM, Italy
  • 4 University of Messina, Department of Veterinary Sciences, Italy

In the last years, metal oxide nanoparticles (NPs) are receiving increasing attention due to their different applications. NPs have unique physicochemical properties that differ substantially from their respective bulk materials of the same composition. According to several studies, titanium dioxide (TiO2) nanoparticles (NPs) have short-term risks on Danio rerio (zebrafish). In the present study, we focus on the potential toxic effects that can be caused by a long-term exposure to titanium dioxide (TiO2) nanoparticles (NPs) on zebrafish. The zebrafish, has long been recognized as a useful model organism in ecotoxicology. Zebrafish Wild-type AB strains, were obtained from the Sicilian Center of Experimental Ichthyiopathology (CISS), University of Messina, Italy, where they were kept in a "Fish facilities" (Stand Alone Unit, Tecniplast), a closed-loop system for the continuous monitoring of vital parameters. Adult specimens of zebrafish were exposed to different concentrations of TiO2 NPs (0,5-1 mg/L) (Degussa P25, Sigma Aldrich), for 90 days. Histological, immunohistochemical and molecular analysis were performed on their gills, gut, skin, muscles and liver. All samples analyzed by immunohistochemical investigation, showed a very clear positive response to Metallothioneins (Abcam, 1:500). Moreover, the positivity was confirmed by Western blot analysis (WB). Histological examination showed intestinal alterations and in particular, partial thinning of the folds and occasional flaking of epithelium and morpho-functional alterations in the gill epithelium. Histological analysis on muscles, liver and skin showed no alterations. In conclusion, the current study confirmed only a slight cytotoxicity of TiO2 NPs on zebrafish adults after a long-term exposure, however, further and wider studies are required to establish their effects on other organs and to evaluate the severity of their toxicity.


This research has been supported by the FP7 European Project WATER (Grant Agreement 316082).

Keywords: Immunohistochemistry, Metallothionein, TiO2 nanoparticles, Zebrafish (Danio rerio), Cytoxicity, western blot

Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015.

Presentation Type: Poster Presentation

Topic: Physiology, Behavior and Toxicology

Citation: Pecoraro R, Camiolo G, Droutsa A, Tibullo D, Buccheri MA, Impellizzeri G, Brundo MV, Marino F and Privitera V (2015). Toxic effects caused by a long-term exposure of Danio rerio to TiO2 nanoparticles.. Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.FMARS.2015.03.00139

Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.

The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.

Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.

For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.

Received: 27 Nov 2015; Published Online: 27 Nov 2015.

* Correspondence: Dr. Roberta Pecoraro, University of Catania (Italy), Department of Biological, Geological and Environmental Sciences, Catania, I-95124, Italy, pecoraro_roberta@libero.it