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Determination of the community-level physiological profiles (CLPP) using BiologTM ECO-plates in the river Minho estuary sediments (Northern Portugal)

Julio C. Lopes1*, Virgílio Peixoto1, Ana Coutinho1, Cláudia Mota1 and Sara Fernandes1
  • 1 Instituto Politécnico de Viana do Castelo, Portugal

Community-level physiological profiling (CLPP) based on sole carbon substrate utilization profiles (CSUP) was first demonstrated by Garland and Mills (1991) using BIOLOG microplates to distinguish between different communities of heterotrophic soil bacteria. The CLPP is a rapid screening method used to characterize microbial communities of different habitats, ranging between sediments to seawater and between oligotrophic groundwater to soil and fertilizers. This method involves the direct inoculation of environmental samples in microtiter plates containing different carbon sources, nutrients and a redox dye, incubation and detection by mass spectrometry of heterotrophic microbial activity. It has the advantage that it is a simple and fast technique to analyze but as the disadvantages that require careful with the data acquisition and the interpretation analysis. CLPP has advantages over both classic cell culturing techniques and molecular level RNA amplification as these other techniques are time consuming and require specialized expertise. BIOLOG EcoPlates™, consisting of 96 well plates containing 31 different carbon sources and a blank in triplicate, have been developed specifically for ecological studies (other BIOLOG plates were originally designed for species identification). Coastal regions in recent decades have been characterized by a high population and urban growth that was aided by the rapid industrial development, which translates into a reduction of the environmental quality of coastal areas. In Portugal, more than 40% of the population is concentrated in the coastal strip, which corresponds to 4 million inhabitants and thus there is a high human pressure on the coastal zone and there are currently 13 estuaries and lagoons: north Tagus (Minho, Cávado, Lima, Ave, Douro, Ria de Aveiro, Mondego, Tagus) and south lies the Sado, Mira, Arade, Ria Formosa and Guadiana. The samples analyzed were collected at 5 sampling points in the estuarine area of the river Minho, in the Portuguese bank. The collected data where used to determine average well colour development (AWCD) and the different AWCD value is used to determine the carbon source utilization and the adjust kinetic parameters (Gompertz model).

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Keywords: CLPP, Gompertz model, Estuaries/Coastal Systems, Minho River, Microbial Diversity, microbial development

Conference: IMMR | International Meeting on Marine Research 2016, Peniche, Portugal, 14 Jul - 15 Jul, 2016.

Presentation Type: Poster presentation

Topic: Biodiversity, Conservation and Coastal Management

Citation: Lopes JC, Peixoto V, Coutinho A, Mota C and Fernandes S (2016). Determination of the community-level physiological profiles (CLPP) using BiologTM ECO-plates in the river Minho estuary sediments (Northern Portugal). Front. Mar. Sci. Conference Abstract: IMMR | International Meeting on Marine Research 2016. doi: 10.3389/conf.FMARS.2016.04.00088

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Received: 14 May 2016; Published Online: 13 Jul 2016.

* Correspondence: Prof. Julio C Lopes, Instituto Politécnico de Viana do Castelo, Ponte de Lima, Portugal, juliocesar@esa.ipvc.pt