Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus
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1
Universitat de Girona, Biology Department, Spain
The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. Six microsatellites departed significantly from Hardy-Weinberg expectations after Bonferroni correction. The 21 polymorphic loci under Hardy-Weinberg equilibrium had a resolving power higher than 97% for parentage studies. These novel microsatellites provide additional tools to address questions relating to diversity, population structure and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource.
Acknowledgements
This work was supported by a grant from Spanish Ministerio de Economia y Competitividad (CTM2014-54648-C2-2-R) and Beca Cooperativa la Equitativa to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014).
Keywords:
Aristeus antennatus,
Next-generation sequencing,
microsatellite loci,
Population Genetics,
blue and red shrimp
Conference:
XIX Iberian Symposium on Marine Biology Studies, Porto, Portugal, 5 Sep - 9 Sep, 2016.
Presentation Type:
Poster Presentation
Topic:
4. FISHERIES, AQUACULTURE AND BIOTECHNOLOGY
Citation:
Heras
S,
Planella Albi
L,
Caldarazzo
I,
Vera
M,
García-Marín
J and
Roldán
M
(2016). Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus.
Front. Mar. Sci.
Conference Abstract:
XIX Iberian Symposium on Marine Biology Studies.
doi: 10.3389/conf.FMARS.2016.05.00216
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Received:
29 Apr 2016;
Published Online:
03 Sep 2016.
*
Correspondence:
Miss. Laia Planella Albi, Universitat de Girona, Biology Department, Girona, Spain, laia.planella@udg.edu