Event Abstract

Pseudomonas aeruginosa in Peniche coastal waters

  • 1 Polytechnic Institute of Leiria, MARE - Marine and Environmental Sciences Centre, Portugal

P. aeruginosa is a ubiquitous organism with minimal requirements for survival and a remarkable ability to adapt to a variety of environmental conditions [1-2]. Members of this species can even survive for months in deionized or distilled water [3]. In the last decades, P. aeruginosa has become one of the most studied bacteria in the public health area due to the fact that a small percentage of strains, demonstrated to be a significant human threat [1]. It is primarily associated with ear, eye and skin infections, causing serious nosocomial infections in cystic fibrosis and burn patients [4-7]. Since the 60’s, that traditional microbiological techniques have been used for the detection and enumeration of P. aeruginosa, mostly the membrane filtration (MF) method, included in the ISO 16266:2006 [8]. Having several limitations, especially the fact that is time consuming, there is an urgent need for the development of alternative methods that overcome the limitations found in conventional methodologies. Pseudalert®/Quanti-Tray® MPN test is a new method, developed by IDEXX Laboratories Inc., which signals the presence of P. aeruginosa in just 24 hours, clearly reducing the time needed to obtain the results. But this methodology is only indicated for tap, pool and drinking water [9-10]. Results showed no significant differences (p-value>0.05) when comparing the new method with the traditional microbiological method, suggesting that Pseudalert®/Quanti-Tray® MPN test can be considered a faster, easier to perform, and safer alternative methodology to assess P. aeruginosa contamination in coastal waters. The major drawback of microbiological methods is the inability to detect viable but non-culturable (VNBC) forms [8]. PCR-based assays have become more attractive, but most authors still use a culture-enrichment step or a short grow step in the culture medium [11-12]. So, we developed a preliminary RT-PCR assay to detect P. aeruginosa without any of these previous steps. The estimated time of the assay was 6 hours, allowing the detection of P. aeruginosa through the amplification of the ecfX gene and using a TZ lysing solution to extract the bacterial genomic DNA of the membrane filters. This assay constitutes a viable alternative to the conventional methodologies, when applied to the detection of P. aeruginosa in seawater samples.

Acknowledgements

This study had financial support of Fundação para a Ciência e Tecnologia (FCT) through the strategic project UID/MAR/04292/2013 granted to MARE.

References

1. Mena KD, Gerba CP. Risk Assessment of Pseudomonas aeruginosa in Water. Reviews of Environmental Contamination and Toxicology, Vol 201 2009;201:71-115. doi:10.1007/978-1-4419-0032-6_3.
2. Hardalo C, Edberg SC. Pseudomonas aeruginosa: Assessment of risk from drinking water. Critical Reviews in Microbiology 1997;23(1):47-75. doi:10.3109/10408419709115130.
3. Wang MC, Liu CY, Shiao AS, Wang T. Ear problems in swimmers. Journal of the Chinese Medical Association 2005;68(8):347-352. doi:10.1016/S1726-4901(09)70174-1.
4. Trautmann M, Lepper PM, Haller M. Ecology of Pseudomonas aeruginosa in the intensive care unit and the evolving role of water outlets as a reservoir of the organism. American Journal of Infection Control 2005;33(5):S41-S49. doi:10.1016/j.ajic.2005.03.006.
5. Asghari FB, Nikaeen M, Mirhendi H. Rapid monitoring of Pseudomonas aeruginosa in hospital water systems: a key priority in prevention of nosocomial infection. Fems Microbiology Letters 2013;343(1):77-81. doi:10.1111/1574-6968.12132.
6. Loveday HP, Wilson JA, Kerr K, Pitchers R, Walker JT, Browne J. Association between healthcare water systems and Pseudomonas aeruginosa infections: a rapid systematic review. Journal of Hospital Infection 2014;86(1):7-15. doi:10.1016/j.jhin.2013.09.010.
7. Directive 2006/7/EC. Directive 2006/7/EC of the European Parliament and of the Council of 15 February 2006 concerning the management of bathing water quality and repealing Directive 76/160/EEC. Official Journal of the European Union 2006;L 064(1882):37-51. doi:L 102/15.
8. Mohammed RL, Echeverry A, Stinson CM, et al. Survival trends of Staphylococcus aureus, Pseudomonas aeruginosa, and Clostridium perfringens in a sandy South Florida beach. Marine Pollution Bulletin 2012;64(6):1201-1209. doi:10.1016/j.marpolbul.2012.03.010.
9. Amagliani G, Parlani ML, Brandi G, Sebastianelli G, Stocchi V, Schiavano GF. Molecular detection of Pseudomonas aeruginosa in recreational water. International Journal of Environmental Health Research 2012;22(1):60-70. doi:10.1080/09603123.2011.588325.
10. Sartory DP, Pauly D, Garrec N, et al. Evaluation of an MPN test for the rapid enumeration of Pseudomonas aeruginosa in hospital waters. Journal of Water and Health 2015;13(2):427-436. doi:10.2166/wh.2014.187.
11. Sartory DP, Brewer M, Beswick A, Steggles D. Evaluation of the Pseudalert®/Quanti-Tray® MPN Test for the Rapid Enumeration of Pseudomonas aeruginosa in Swimming Pool and Spa Pool Waters. Current Microbiology 2015;71(6):699-705. doi:10.1007/s00284-015-0905-8.

Keywords: P. aeruginosa, Water Quality, water safety, coastal bathing waters, Membrane Filtration, Pseudalert®/Quanti-Tray® MPN test

Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018.

Presentation Type: Oral Presentation

Topic: Blue Biotech

Citation: Januário AP, Afonso CN, Mendes SL and Rodrigues MJ (2019). Pseudomonas aeruginosa in Peniche coastal waters. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00002

Received: 06 May 2018; Published Online: 07 Jan 2019.

* Correspondence: Ms. Adriana P Januário, Polytechnic Institute of Leiria, MARE - Marine and Environmental Sciences Centre, Leiria, Portugal, adrianajanuario13@gmail.com

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