Bioprinting 3D functional neural tissue using human neural and induced pluripotent stem cells
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1
University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, Australia
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2
Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Stem Cell and Reproductive Biology, China
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3
The University of Melbourne, Department of Medicine, St Vincent’s Hospital, Australia
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4
The University of Melbourne, Department of Surgery, St Vincent’s Hospital, Australia
Printing stem cells with biomaterials is a promising strategy to engineer tissue for in vitro modelling and regenerative medicine[1]. Here we report a simple and reproducible approach to constructing neural tissue by printing human neural or induced pluripotent stem cells that are differentiated in situ to functional neurons and supporting neuroglia. The supporting biomaterial incorporates a novel clinically relevant gel-based bioink comprising alginate, carboxymethyl-chitosan and agarose. The printed bioink rapidly gels by stable crosslinking to form a porous 3D scaffold encapsulating stem cells for subsequent expansion and differentiation.
Differentiated neurons are spontaneously active, show a bicuculline-induced increased calcium response, and are predominantly gamma-aminobutyric acid expressing.
The 3D tissues will enable interrogation of neural development, function and disease, and may be adaptable for generating other neuronal and non-neuronal tissue subtypes in vitro.
References:
[1] Murphy, S.V. and A. Atala, 3D bioprinting of tissues and organs. Nat Biotechnol, 2014. 32(8): p. 773-785.
Keywords:
stem cell,
Functionalization,
3D scaffold
Conference:
10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016.
Presentation Type:
Poster
Topic:
Biomimetic materials
Citation:
Gu
Q,
Tomaskovic-Crook
E,
Kapsa
R,
Cook
M,
Zhou
Q,
Wallace
G and
Crook
J
(2016). Bioprinting 3D functional neural tissue using human neural and induced pluripotent stem cells.
Front. Bioeng. Biotechnol.
Conference Abstract:
10th World Biomaterials Congress.
doi: 10.3389/conf.FBIOE.2016.01.01087
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Received:
27 Mar 2016;
Published Online:
30 Mar 2016.
*
Correspondence:
Dr. Qi Gu, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, qg863@uowmail.edu.au
Dr. Rob Kapsa, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, Email1
Dr. Mark Cook, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, Email2
Dr. Qi Zhou, Institute of Zoology, Chinese Academy of Sciences, State Key Laboratory of Stem Cell and Reproductive Biology, Beijing, China, qzhou@ioz.ac.cn
Dr. Jeremy Crook, University of Wollongong, ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, New South Wales, Australia, jcrook@uow.edu.au