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Optimization of a Surface plasmon resonance sensor platform for point of care testing

Franklin Iheanacho1*, Ornella Sathoud1 and Karl Booksh1
  • 1 University of Delaware, United States

Point-of-care testing (POCT) is diagnostic testing performed in a short time and by the patient’s bedside. POCT is typically performed by non-laboratory personnel with the main goal to perform convenient and fast tests to medical professionals. The results are used for clinical decision-making to support patients’ diagnosis. Several biosensors used as POCT utilizes antibody-antigen interaction as part of the sensing system. The goal of this project is to use surface plasmon resonance (SPR) as the detection technique for an in-laboratory developed microfluidic sensor to monitor binding events on the sensing surface. The sensing surface is a 50 nm gold layer. X-ray photoelectron spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectroscopy (TOF-SIMS) were used to investigate the successful grafting of the self-assembled monolayer (SAM) layer 4-aminophenylalanine, deposited prior proteins immobilization. 4-aminophenylalanine is used as the linker to allow receptors immobilization. The sensor surface characterization was attempted with SPR, Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy, to investigate the successful immobilization of bovine serum albumin and anti-bovine serum albumin after the electrografting of the SAM layer. Air bubbles removal in the microfluidic system using the surfactants Tween and Sodium dodecylbenzene sulfonate (SDBS) was investigated as well in this report.

Acknowledgements

Ornella Sathoud, Ph.D Karl Booksh, Ph.D Rosalind Johnson, Ph.D University of Delaware NOBCChE Advancing Science Conference Grant

References

Admin. “Advancing Microfluidics Research Using High-Speed Cameras.” Accessed September 26, 2018. https://www.cytofluidix.com/advancing-microfluidics-research-using-high-speed-cameras/. Garstecki, P, A M Gañán-Calvo, and G M Whitesides. “Formation of Bubbles and Droplets in Microfluidic Systems.” Bulletin of the Polish Academy of Sciences 53, no. 4 (2005): 12. Wang, K., Y. C. Lu, J. H. Xu, and G. S. Luo. “Determination of Dynamic Interfacial Tension and Its Effect on Droplet Formation in the T-Shaped Microdispersion Process.” Langmuir 25, no. 4 (February 17, 2009): 2153–58. https://doi.org/10.1021/la803049s.

Keywords: Surface plamon resonance, point of care (POC) troponin, Microfludic chip, surfactance influence, spectrosccopy, Clinical test development, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, hydrochloride), EDC-NHS, EDC-NHS cross-linking, SPR (Surface Plasmon Resonance), PoC (point of care), POCT (point-of-care testing), Lab on a chip, Lab on a Chip and Microfluidic Devices, Air bubble, Air bubble removal, bubbles, Air bubbles concentration, Micelle, Micelle concentration, Electrografting of diazonium salt, Antibody immobilization, Heart attack and stroke, acute mycardial infarction, Time of Flight (ToF), TOF SIMS, Time of flight secondary ion mass spectrometer (TOF-SIMS)

Conference: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference , Orlando, Florida, United States, 17 Sep - 20 Sep, 2018.

Presentation Type: Oral Presentation

Topic: Analytical Chemsitry

Citation: Iheanacho F, Sathoud O and Booksh K (2019). Optimization of a Surface plasmon resonance sensor platform for point of care testing. Front. Chem. Conference Abstract: National Organization for the Professional Advancement of Black Chemists and Chemical Engineers (NOBCChE) 45th Annual Conference . doi: 10.3389/conf.fchem.2018.01.00007

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Received: 26 Sep 2018; Published Online: 17 Jan 2019.

* Correspondence: Mr. Franklin Iheanacho, University of Delaware, Newark, Delaware, 19716, United States, franki@udel.edu