Neuroanatomical localization of kiss ligands and receptors in zebrafish brain
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1
University of Rennes, Beaulieu Campus, France
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2
INRA, Physiologie de la Reproduction et des Comportements,, France
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3
Monash University, School of Medicine and Health Sciences, Malaysia
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4
Korea University, Graduate School of Medicine, Republic of Korea
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5
European Institute for Peptide Research, Neuronal and Neuroendocrine Differentiation and Communication, France
Kisspeptins, the products of the KISS1 gene, have recently emerged as key players in the regulation of the reproductive axis in mammals. They stimulate GnRH release at the hypothalamic level through the receptor KISS1R. Due to genome duplication and gene loss events, up to three isoforms of both kisspeptin ligands and receptors have been reported in non-mammalian vertebrates. In teleost fish, which have two kiss and two kiss receptors genes, the anatomical characterization of these systems remains elusive and their interactions with the GnRH systems are not established. This study aimed at defining the anatomical localization of kiss1 and kiss2-expressing cells as well as that of the receptors kiss1r and kiss2r by in situ hybridization in adult zebrafish brain. Our findings show two separated neuronal systems: kiss1 neurons were only localized in the habenular region, whereas kiss 2-expressing cells were mostly observed in the mediobasal hypothalamus. In addition, the organization and projections of kiss1- and kiss2-containing cells were studied by immunohistochemistry using specific antibodies generated against the precursors of the zebrafish kiss isoforms. Lastly, the potential relationships between the kiss and GnRH systems, as well as estrogen and leptin receptors are discussed. Altogether, our results provide new evidence for the involvement of the kiss systems in the modulation of the reproductive axis in zebrafish.
Acknowledgements
Supported by the EU Project LIFECYCLE (FP7-222719) and the NEMO project
Keywords:
comparative endocrinology
Conference:
25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010.
Presentation Type:
Conference Presentation
Topic:
Comparative endocrinology
Citation:
Servili
A,
Le Page
Y,
Caraty
A,
Parhar
I,
Seong
J,
Leprince
J,
Vaudry
H and
Kah
O
(2010). Neuroanatomical localization of kiss ligands and receptors in zebrafish brain.
Front. Endocrinol.
Conference Abstract:
25th Conference of the European Comparative Endocrinologists.
doi: 10.3389/conf.fendo.2010.01.00054
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Received:
26 Aug 2010;
Published Online:
29 Aug 2010.
*
Correspondence:
Dr. Arianna Servili, University of Rennes, Beaulieu Campus, Rennes, France, arianna.servili@univ-rennes1.fr