ACTIVITY OF STEROID-CONJUGATING ENZYMES IN HATCHLING RED-EARED SLIDER TURTLES EXPOSED TO BISPHENOL-A
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1
Illinois State University, School of Biological Sciences, United States
Endocrine disrupting chemicals (EDCs) are a class of compounds that impact the functioning of the endocrine system. To more fully understand how EDCs affect an organism, it is important to study not only the endpoint effects of a compound, but also the transient physiological response of the organism to exposure such as the upregulation of sulfotransferases (SULT) and glucuronyltransferases (UGT), two superfamilies of hepatic enzymes. In vertebrates, SULT and UGT are the two main families of enzymes involved in phase II steroid metabolism and these same enzymes are also utilized by the body for clearance and removal of xenobiotics such as EDCs. This study aims to understand which detoxifying pathway is more prominent in EDC metabolism and whether or not the activity of these enzymes is affected by exposure to an EDC. To investigate this aim, red-eared slider (Trachemys scripta) hatchlings were injected with either Bisphenol-A or a vehicle control and livers were harvested 12, 24, or 48 hours post-injection. Liver homogenates (S9 fraction) were used for enzyme assays of SULT and UGT to quantify and compare enzyme activity. Preliminary analyses show a trend for increased enzyme activity of both SULT and UGT by 24 hours post-injection, however there appears to be a great deal of individual variation in ability to respond to exposure through increased enzyme activity. Investigating changes in enzyme activity will provide insight into mechanisms of endocrine disruption, allowing for a more complete understanding of potential threats of endocrine disruptors on humans and wildlife.
Acknowledgements
Supported by EPA STAR fellowship F09G11129 to S.G.C.
This poster was developed under STAR Fellowship Assistance Agreement no. F09G11129 awarded by the U.S. Environmental Protection Agency (EPA). It has not been formally reviewed by EPA. The views expressed in this presentation are solely those of SGC and the EPA does not endorse any products or commercial services mentioned in this poster
Keywords:
bisphenol A,
endocrine disruption,
enzyme activity,
glucuronyltransferase,
Red-eared slider,
sulfotransferase
Conference:
ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, Ann Arbor, United States, 11 Jul - 13 Jul, 2011.
Presentation Type:
Poster
Topic:
Endocrine disruption
Citation:
Clairardin
S,
Paitz
R and
Bowden
R
(2011). ACTIVITY OF STEROID-CONJUGATING ENZYMES IN HATCHLING RED-EARED SLIDER TURTLES EXPOSED TO BISPHENOL-A.
Front. Endocrinol.
Conference Abstract:
ISAREN 2011: 7th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology.
doi: 10.3389/conf.fendo.2011.03.00005
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Received:
20 Jul 2011;
Published Online:
09 Aug 2011.
*
Correspondence:
Ms. Sandrine Clairardin, Illinois State University, School of Biological Sciences, Normal, IL, United States, sgclair@ilstu.edu