CRUSTACEAN CARDIOACIVE PEPTIDE, ITS RECEPTOR, AND PHYSIOLOGICAL EFFECTS, IN THE BLOOD-GORGING BUG, RHODNIUS PROLIXUS
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1
University of Toronto Mississauga, Department of Biology, Canada
Crustacean cardioactive peptide (CCAP), a cyclic nonapeptide (PFCNAFTGCamide), has multifunctional roles in insects including the stimulation of visceral and cardiac muscle contraction, and the regulation of ecdysis. Previously, we have cloned the cDNA sequence of the CCAP gene from Rhodnius prolixus central nervous system (CNS), and shown its expression in the CNS using in situ hybridization. In the current study, we have verified the amino acid sequence of CCAP in R. prolixus CNS by matrix-assisted laser desorption/ionisation (MALDI) time-of-flight (TOF) mass spectrometry (MS) and MALDI-TOF MS/MS, and shown its distribution in the CNS and peripheral tissues using immunohistochemistry. We have also partially sequenced the cDNA of the RhoprCCAP G-protein coupled receptor (GPCR) and examined its expression in the CNS as well as peripheral tissues of R. prolixus. Physiologically, CCAP dose-dependently stimulates R. prolixus hindgut contractions, with threshold at 5x10-9 M and maximum response at10-7M CCAP. Also, CCAP increases heartbeat frequency in a reversible, dose-dependent manner, with threshold close to 10-11M and maximum response at 10-10M CCAP. CCAP is therefore present in R. prolixus, and acts via GPCRs to modify visceral and cardiac muscle contraction.
Acknowledgements
The authors wish to thank Dr. Li Zhang for assistance in the mass spectrometry analysis. This work was supported by the Natural Sciences and Engineering Research Council of Canada.
Keywords:
Heart,
Hindgut,
insect,
Neuropeptide,
receptor
Conference:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011.
Presentation Type:
Poster
Topic:
General neuroendocrinology
Citation:
Lee
D,
Orchard
I and
Lange
A
(2011). CRUSTACEAN CARDIOACIVE PEPTIDE, ITS RECEPTOR, AND PHYSIOLOGICAL EFFECTS, IN THE BLOOD-GORGING BUG, RHODNIUS PROLIXUS.
Front. Endocrinol.
Conference Abstract:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology.
doi: 10.3389/conf.fendo.2011.04.00029
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Received:
12 Jul 2011;
Published Online:
09 Aug 2011.
*
Correspondence:
Ms. Do Hee Lee, University of Toronto Mississauga, Department of Biology, Mississauga, Ontario, L5L 1C6, Canada, dohee.lee@utoronto.ca