Molecular Cloning of Hepatic Insulin-like Growth Factor-1 cDNA and Sequence Analysis in Lizards
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1
California State University, Fresno, Department of Biology, United States
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2
Rutgers, The State University of New Jersey, Graduate Program in Endocrinology and Animal Biosciences, United States
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3
Rutgers, The State University of New Jersey, Department of Ecology, Evolution, and Natural Resources, United States
The insulin-like growth factor (IGF) hormone family is well conserved in structure and function across all vertebrates. This high degree of conservation is likely due to the critical roles that these hormones play in development, growth, and metabolism. To date, the structure and function of insulin-like growth factors have been described in a wide range of vertebrate taxonomic groups, however, lizards have received little attention. Therefore, the objective of this study was to indentify IGF-1 cDNA sequence data from five lizard species. Primers designed against conserved regions of IGF-1 were used to generate cDNA from Sceloporus undulatus, S. jarrovii, Anolis sagrei, Coleonyx elegans, and Goniurosaurus lichtenfelderi. The cDNA was cloned into the pCR™4-TOPO® TA vector and sequenced. The resulting cDNA base sequences were converted to amino acid (AA) sequences. Sceloporus undulatus IGF-1 contained 55 AA and S. jarrovii, A. sagrei, C. elegans, and G. lichtenfelderi IGF-1 contained 43 AA. Comparison of the deduced AA sequences of IGF-1 to human IGF-1 confirms high sequence identity: S. undulatus (80%), S. jarrovii (74%), A. sagrei (72%), C. elegans (74%), and G. lichtenfelderi (77%). High IGF-1 sequence identity was observed between these species of lizards (86-100%). Although these species belong to the same order, variation occurred in the C domain, which is likely due to the fact that Iguania and Gekkota diverged approximately 240 million years ago. The percent of sequence identity is associated with the degree of relatedness between species and progressively decreases with evolutionary distance. To our knowledge, we are the first to clone IGF-1 from any species of reptile. The partial IGF-1 sequences cloned from lizards contribute to the growing body of literature that indicates that the high percent of sequence identity is associated with the essential involvement of IGF-1 in growth regulatory mechanisms among vertebrates.
Acknowledgements
Supported by the Society for Integrative and Comparative Biology, Graduate School-New Brunswick, and School of Environmental and Biological Sciences.
Keywords:
cloning,
IGF,
IGF-1,
Lizard,
Sequencing
Conference:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology, Ann Arbor, United States, 13 Jul - 16 Jul, 2011.
Presentation Type:
Poster
Topic:
Growth and aging
Citation:
Duncan
CA and
John-Alder
HB
(2011). Molecular Cloning of Hepatic Insulin-like Growth Factor-1 cDNA and Sequence Analysis in Lizards.
Front. Endocrinol.
Conference Abstract:
NASCE 2011: The inaugural meeting of the North American Society for Comparative Endocrinology.
doi: 10.3389/conf.fendo.2011.04.00130
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Received:
19 Jul 2011;
Published Online:
09 Aug 2011.
*
Correspondence:
Dr. Christine A Duncan, California State University, Fresno, Department of Biology, Fresno, CA, 93740, United States, cduncan@csufresno.edu