Comet assay on mice testicular cells
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1
Technical University of Denmark, Division of Toxicology & Risk Assessment, Denmark
Heritable mutations may result in a variety of adverse outcomes including genetic disease in the offspring. In recent years the focus on germ cell mutagenicity has increased and the “Globally Harmonized System of Classification and Labelling of Chemicals (GHS)” has published classification criteria for germ cell mutagens (Speit et al., 2009). The in vivo Comet assay is considered a useful tool for investigating germ cell genotoxicity. In the present study DNA strand breaks in testicular cells of mice were investigated. Different classes of chemicals were tested in order to evaluate the sensitivity of the comet assay in testicular cells. The chemicals included environmentally relevant substances such as Bisphenol A, PFOS and Tetrabrombisphenol A. Statistical power calculations will be presented to aid in the design of future Comet assay studies on testicular cells. Power curves were provided with different fold changes in % tail DNA, different number of cells scored and different number of gels (Hansen et al., 2014). An example is shown in Figure 1. A high throughput version of the Comet assay was used. Samples were scored with a fully automatic comet assay scoring system that provided faster scoring of randomly selected cells.
Acknowledgements
Acknowledgements
Merethe Kjær Hansen1, Murat Kulachia1 and 3, Marianne Dybdahl2, Julie Boberg2 and Vivian Jørgensen2.
1 Technical University of Denmark, Dept. of Applied Mathematics and Computer Science.
2 Technical University of Denmark, National Food Institute.
3 Luleå University of Technology, Dept.of Business Administration, Technology and Social Sciences.
References
Speit, G., Vasquez, M., Hartmann, A. The comet assay as an indicator test for germ cell genotoxicity. Mutation Research/Reviews in Mutation Research. 2009 (681) 3-12.
Hansen, M.K., Sharma, A.K., Dybdahl, M., Boberg, J., Kulahci, M. In vivo Comet assay - statistical analysis and power calculations of mice testicular cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2014. (774) 29-40.
Keywords:
in vivo,
Testis,
High-Throughput Screening Assays,
DNA strand breaks,
Power calculation,
Statistical issues
Conference:
ICAW 2015 - 11th International Comet Assay Workshop, Antwerpen, Belgium, 1 Sep - 4 Sep, 2015.
Presentation Type:
Oral Presentation
Topic:
Statistical issues within comet assay studies
Citation:
Sharma
AK
(2015). Comet assay on mice testicular cells.
Front. Genet.
Conference Abstract:
ICAW 2015 - 11th International Comet Assay Workshop.
doi: 10.3389/conf.fgene.2015.01.00025
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Received:
02 Jun 2015;
Published Online:
23 Jun 2015.
*
Correspondence:
PhD. Anoop K Sharma, Technical University of Denmark, Division of Toxicology & Risk Assessment, Søborg, 2860, Denmark, aksha@food.dtu.dk