T-lymphocytes effectors/memory cells detection by intracellular cytokines staining in children of different age and adults
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1
Clinical and Diagnostic centre, Instite of immunology and physiology of the Ural branch of Russian Academy of Sciences, Russia
The method of intracellular marker cytokines staining was applied in order to quantify normal levels of Th1, Tc1, Th2, Tc2, Th17, Tnc17 subpopulations in children of different age and in adults. The studied group consisted of 150 children and adolescents aged 7 months - 18 years and 45 adults of 25-45 years of age. It has been found that Th1 and Tc1-lymphocytes absolute and relative levels significantly lower in infants of 7-12 months of age than in adults. Simultaneously the Th2 lymphocytes count was similar in 7-12 months old infants and adults, whilst Tc2-lymphocytes absolute count was higher in 7-12 months old infants than in all other age groups. Th17, Tnc17 lymphocytes absolute counts were higher in 7-12 months old infants than in adults. Thus, Th2(Tc2)/Th17(Tnc17) immune response polarization was found in 7-12 months old infants which possibly helps Th2-mediated allergic disorders manifestation in atopy predisposed infants. Th1(Тс1) and Th2(Тс2) lymphocytes absolute counts were not different significantly in children older than 12 months old and in adults. Th2-lymphocytes levels decreasing and IL17А-positive cells levels increasing trends were identified in 15-18 years old adolescent group. This phenomenon can mediate the induction of autoimmune disorders predisposition at this age. Th1/Th2 counts proportion and Tc1/Tc2 counts proportion were 18.7 and 56.8, respectively in adults of 25-45 years of age. Сorrelations analysis identified the strongest correlation between Th1/Тс1 subpopulations and memory cells. Relative increase in Th1 и Тс1 lymphocytes portion linked to T-memory cells pool formation reflects immune system maturation within the infants aging.
Keywords:
T cell,
Intracellular cytokines,
Children and adolescents,
Adult,
T cell memory
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Lagereva
J
(2013). T-lymphocytes effectors/memory cells detection by intracellular cytokines staining in children of different age and adults.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00018
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Received:
09 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Julia Lagereva, Clinical and Diagnostic centre, Instite of immunology and physiology of the Ural branch of Russian Academy of Sciences, Ekaterinburg, Russia, anna-lagereva@yandex.ru