Combined detection of IFNγ, TGFα and IL-6 cаn discriminate between active TB disease and latent infection
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1
St. Petersburg Pasteur Institute, Russia
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2
Central Tuberculosis Research Institute, Russia
Interferon gamma release assays are used for diagnosing Mycobacterium tuberculosis ( M.tb.) infection. However these tests fail to differentiate active TB from latent TB infection (LTBI).
Aim: Selection of informative biomarkers for the diagnosing M.tb. infection and for discriminating between latent TB infection (LTBI) and active TB disease.
Materials and methods: We recruited 54 TB patients, 47 TB contacts and 43 healthy donors and performed the “QuantiFERON-TB Gold In-Tube” (“Cellestis”, Аustralia). Using XMap technology levels of 10 cytokines (EGF, MIP-1β, VEGF, IL-2, IL-4, IL-6, IL-1α, IFN-α2, TGFα, TNFα) as well as sIL-2Rα and sCD40L were evaluated in unstimulated (NIL) and Mycobacterium tuberculosis specific antigen (AG) stimulated plasma. We also determined levels of IP-10 in 48 patients by ELISA.
Results: Six out of 13 biomarkers distinguished active TB from LTBI . As a result of making “ decision tree” in JMP 9.0 software, we choose three the most informative cytokines. The combination between IFNγ, TGFα and IL-6 may possible to distinguish active TB from latent infection with sensitivity 96,3% and specifity 80,7% (AUC=0,9). We also observed very high levels of IP-10 and IL-2, which correlated with IFNγ levels (r=0,71 and 0,79 respectively).
Conclusion: IL-2 or IP-10 as well as IFNγ, detection could be used in a first step to diagnose ( M.tb.) infection. A second step of the test could be performed if positive IL-2 / IP-10 / IFNγ results are obtained to measure three-marker combination of IFNγ, TGFα and IL-6 to differentiate individuals with active TB from LTBI.
Keywords:
latent infection,
IFNγ,
xMap,
QuantiFERON-TB Gold In-Tube,
pulmonary tuberculosis
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Vasilyeva
EV,
Verbov
VN,
Nikitina
IY,
Lyadova
IV and
Totolian
AA
(2013). Combined detection of IFNγ, TGFα and IL-6 cаn discriminate between active TB disease and latent infection.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00030
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Received:
08 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Miss. Elena V Vasilyeva, St. Petersburg Pasteur Institute, St. Petersburg, Russia, ev_vasilyeva@pasteurorg.ru