Antibodies to citrullinated peptide in children with juvenile arthritis.
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1
Institute of immunology and physiology, Russia
Detection of antibodies to citrullinated peptides is widely used for diagnostics of rheumatoid arthritis in adults and demonstrates high sensitivity and specificity. Using of these tests is much less common in the diagnostics of juvenile arthritis (JA). The aim of our work was to investigate the frequency of anti-mutated citrullinated vimentin (anti-MCV) and anti-cyclic citrullinated peptide (anti-CCP) antibodies in children with juvenile arthritis.
The levels anti-MCV (Orgentec, Germany) and anti-CCP (Euroimmun, Germany) antibodies were determined by ELISA in 219 children with juvenile arthritis and in 25 healthy children.
Anti-MCV antibodies were found in 12% of children with oligoarticular JA (n = 114), 19% with polyarticular JA (n = 79), and 23% with systemic onset of disease (n = 26). The total frequency of anti-MCV antibodies was 18%, thus, the test sensitivity is low for the diagnostics of juvenile arthritis. High levels of anti-MCV antibodies were detected in 2 of 25 healthy children.
A simultaneous determination anti-MCV and anti-CCP antibody was held in 124 children with juvenile arthritis. Using the manufacturer's cut-off threshold 20U/ml for anti-MCV antibodies 19 children had concentrations of anti-MCV antibodies above normal, high levels of anti-CCP antibodies were observed in 11 ones. Increasing cut-off for anti-MCV antibodies up to 30U/ml the positive results were only in 11 patients, which shows the specificity of laboratory tests became equal. The concentration of anti-MCV antibodies was below 30U/ml in all healthy children. Thus, the cut-off for anti-MCV antibodies should be specified for the diagnostics of juvenile arthritis.
Keywords:
juvenile arthritis,
citrullinated peptides,
Autoantibodies,
Children,
anti-MCV
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Pashnina
I and
Krivolapova
I
(2013). Antibodies to citrullinated peptide in children with juvenile arthritis..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00071
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Received:
09 Mar 2013;
Published Online:
22 Aug 2013.
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Correspondence:
Dr. Irina Pashnina, Institute of immunology and physiology, Ekaterinburg, Russia, i.pashnina@yandex.ru