Cytokines in relation to autoantibodies before onset of symptoms in systemic lupus erythematosus (SLE)
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1
University Umeå, Clinical immunology/Clinical Microbiology, Sweden
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2
University Umeå, Department of Public Health and Clinical Medicine/Rheumatology, Sweden
Background
This study was performed in order to analyse cytokines in blood, and their relationship to autoantibodies in individuals who later developed SLE
Methods
Thirty-five patients with SLE (ACR criteria) who had donated blood to the Medical Biobank, Umeå, Sweden, before onset of symptoms, and 140 age- and sex-matched controls from the same biobank.were analysed for interferon (IFN)-α, interleukin (IL)-4, IL-9, IL-10, interferon inducible protein 10 (CXCL10/IP-10) and monocyte chemotactic protein-1 (CCL2/MCP-1), using a multiplex method. The associations of these cytokines to autoantibodies (ANA, ENA, anti-dsDNA and anti-histones) from the same blood samples (analysed earlier) were evaluated.
Results
The IP-10 levels were higher in the presymptomatic individuals than in controls, p=0.02, and had the most obvious association to autoantibodies, significantly correlated with ANA, SSA, Jo-1 and to autoantibody positivity over all (p=0.03, 0.01, 0.03 and <0.001 respectively). MCP-1 correlated with SSA, SSB and antibody positivity over all (p=0.009, 0.047 and 0.015 respectively), IFN-α with anti-SSB and antibody positivity over all (p=0.027 and 0.025). The levels of IFN-α, IP-10 and MCP-1 correlated with the number of autoantibodies in presymptomatic individuals (p= 0.066, <0.01 and 0.015 respectively). IL-10, IP-10 and MCP-1 increased between the presymptomatic sample and after SLE diagnose, (p=0.026, <0.0001 and <0.0001 respectively).
Conclusions
IP-10, MCP-1 and IFN-α had the clearest correlation with autoantibody formation before disease onset of SLE. Since secretion of IP-10 and MCP-1 are mediated by IFN- α, these findings support previous theories that the IFN-system is important in the early SLE pathogenesis and autoantibody formation.
Keywords:
systemic lupus erythematosus (SLE),
Autoantibodies,
Cytokines,
Pathogenesis,
predating
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Eriksson
C and
Rantapää Dahlqvist
S
(2013). Cytokines in relation to autoantibodies before onset of symptoms in systemic lupus erythematosus (SLE).
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00156
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Received:
11 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Catharina Eriksson, University Umeå, Clinical immunology/Clinical Microbiology, Umeå, Sweden, eriksson.ca@gmail.com