Domination of Th17 subpopulation of lymphocytes during tumor progression.
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1
Tehran university of medical sciences, Immunology, Iran
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2
Shahid Sadoughi university of medical sciences, Immunology, Iran
Background:
Inflammatory responses play a decisive role in the early stages of tumor development, Progress, Invasion and metastasis. An inflammatory mechanism is also effective in immune surveillance and efficacy of the treatment. The tumor microenvironment consists of a complex network of various cell types including tumor-infiltrating lymphocytes. Tumor infiltrating lymphocyte, have a vast and dynamic relationship with the cancer cells. Similar to human, Th17 cells are found to infiltrate tumors in murine models of cancer. Th17 cells are known with potent pro-inflammatory activities, which are mainly mediated by its cytokines. The purpose of this study was to explore whether there is a linkage between the infiltration of Th17 cells and tumor progression in an experimental model of murine melanoma.
METHODS:
After induction of tumors in mice using B16F10 melanoma cell line subcutaneously, tumors and draining lymph nodes were extracts at 3 different time points during tumor progression. We quantified expression pattern of functional group of genes associated with Th17 Using Real-time PCR.
RESULTS:
Real-time PCR analysis of gene expression in tumor microenvironment and draining lymph node revealed increased expression of related genes, suggesting significant increase in Th17 infiltrating cell during tumor progression.
CONCLUSIONS:
Along with the increase of Th17 cells, a pro inflammatory microenvironment is performed, which contributed to the tumor progression. This tumor is characterized by a Proinflammatory cytokine microenvironment. Evaluation of different subpopulation of T lymphocytes infiltrating the tumor site could be a valuable prognostic factor showing tumor progression and novel therapeutic strategies could be designed to change the microenvironment.
Keywords:
Tumor Microenvironment,
Inflammation,
T helper17,
Gene Expression,
Real-Time PCR,
Murine model of melanoma
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Ajami
M,
Mansouri
R and
Hadjati
J
(2013). Domination of Th17 subpopulation of lymphocytes during tumor progression..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00201
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Received:
13 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Jamshid Hadjati, Tehran university of medical sciences, Immunology, Tehran, Iran, hajatij@sina.tums.ac.ir