Novel monoclonal antibodies against human Carbonic Anhydrase XII, a potential biomarker of tumor cells
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1
Institute of Biotechnology, Vilnius University, Department of Immunology and Cell Biology, Lithuania
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2
Institute of Biotechnology, Vilnius University, Department of Biothermodynamics and Drug Design, Lithuania
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3
Institute of Biomedical Technology, University of Tampere, Finland
Carbonic anhydrases (CAs, EC 4.2.1.1) are zinc enzymes that catalyse the reversible hydration of carbon dioxide to bicarbonate. CAs have important physiological and pathological functions in human organism. CA IX is well-recognized tumor marker. Recently, the association of CA XII with human cancers has been demonstrated. The CA XII is a transmembrane protein with an extracellular catalytic domain. It is involved in tumor progression by acidification of the extracellular milieu and regulation of intracellular pH. Recent studies indicate that CA XII is aberrantly overexpressed in breast, cervix, brain cancers, renal carcinomas. Therefore, CA XII might be considered as a useful biomarker of tumor cells and a promising target for specific therapies. The aim of the current study was to develop new monoclonal antibodies (MAbs) against human recombinant CA XII and evaluate their diagnostic potential.
Seven stable hybridoma cell lines producing IgG antibodies against human CA XII were generated. The MAbs were highly specific to CA XII and did not cross-react with other human recombinant CAs. In order to demonstrate the diagnostic value of the MAbs, they were employed for the IHC staining of CA XII in human tumor tissue specimens. The MAbs demonstrated a strong and specific reaction with colon and renal carcinoma specimens and did not show any unspecific background staining of the respective normal tissues. Flow cytometry analysis revealed a specific immunostaining of CA XII in human tumor cell lines.
The newly developed MAbs represent a promising diagnostic tool for the immunodetection of CA XII-expressing tumor cells.
Keywords:
Diagnostic Imaging,
Immunohistochemistry,
carbonic anhydrase XII,
Monoclonal antibody,
cancer biomarker
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Dekaminaviciute
D,
Lasickiene
R,
Zilnyte
M,
Jogaite
V,
Michailoviene
V,
Parkkila
S,
Matulis
D and
Zvirbliene
A
(2013). Novel monoclonal antibodies against human Carbonic Anhydrase XII, a potential biomarker of tumor cells.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00210
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Received:
12 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Dovile Dekaminaviciute, Institute of Biotechnology, Vilnius University, Department of Immunology and Cell Biology, Vilnius, Lithuania, dovile.dekaminaviciute@bti.vu.lt