Association of -1082 A/G promoter polymorphism of IL-10 gene with colorectal cancer development
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1
Trakia University, Medical Faculty, Department of Neurosurgery, Surgery and Urology, Bulgaria
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2
Trakia University, Medical Faculty, Department of Molecular Biology, Immunology and Medical genetics, Bulgaria
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3
University School of Medicine, Oncology Center,, Bulgaria
The cancer-associated inflammation and anti-tumor immune response to colorectal carcinoma (CRC) is regulated by cytokines produced from activated immune cells. IL-10 is a Treg cytokine suppressing both Th1 immune response and anti-tumor immunity in hosts. The role of functional polymorphism of IL-10 gene in CRC development still remains elusive.
This study was designed to compare -1082 A/G IL10 genotype distribution in 119 CRC patients to a group of 154 matched healthy donors using ARMS-PCR assay. We also investigated serum IL-10 levels in an association to genotype by ELISA. In the study population the slightly enhanced frequency of homozygous genotype GG (18% vs 13%; OR=1.414; 95%CI=0.64÷3.11; p=0.347) was seen in cases versus controls. When CRC patient’s group was divided into stages of disease by TNM classification we observed higher risk of advanced CRC (III-IV stages) for individuals with GG genotype: OR=2.229; 95%CI= 0.828÷6.018; p=0.077. G allele was also overrepresented in advanced vs early CRC (OR=1.581; 95% CI=0.912÷2.743; p=0.082). According to the IL-10 serum levels, CRC patients with GG genotype produced higher IL-10 than AA and AG patients in early stages, similar to healthy control. Moreover, advanced cancer patients with AA and AG genotype produced significantly higher IL-10 compared to patients in early stage (p=0.002 for AA; p= 0.007 for AG genotype), whereas for advanced cancer patients with GG genotype this difference does not reach statistical significance. In conclusion, this study demonstrated that -1082 A/G polymorphism of IL-10 gene has a role in CRC progression rather then CRC genetic predisposition.
Keywords:
colorectal cancer,
IL-10,
promoter polymorphism,
-1082A>GIL10,
Cytokines
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Stanilov
NS,
Dobreva
ZG,
Jovchev
J,
Deliysky
T,
Avramova
A,
Miteva
L and
Stanilova
SA
(2013). Association of -1082 A/G promoter polymorphism of IL-10 gene with colorectal cancer development.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00213
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Received:
14 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Noyko S Stanilov, Trakia University, Medical Faculty, Department of Neurosurgery, Surgery and Urology, Stara Zagora, 6000, Bulgaria, noyko.stanilov@gmail.com