Effect of static magnetic field on pollen-induced allergic airway inflammation in a murine model
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1
University of Debrecen, Department of Immunology, Hungary
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2
University of Debrecen, Department of Computer Science, Hungary
It has been demonstrated that pollen NAD(P)H oxidases generate oxidative stress in the airway epithelium and this immediate oxidative insult is crucial for the development of allergen-driven airway inflammation. In this study we aimed to define the effect of inhomogeneous static magnetic field (iSMF) on pollen-induced allergic airway inflammation since several lines of evidence suggest that iSMF is able to trigger biological responses at least partly through free radical reactions. BALB/c mice were sensitized by two intraperitoneal injection of ragweed pollen extract (RWE) and challenged with RWE intranasally. Inflammation was evaluated by determining inflammatory cell accumulation and mucin levels, as well as histological analysis of the airways. iSMF was generated with an apparatus optimized to small experimental animals. We found that iSMF did not affect the sensitization phase of the allergic responses; however, even a single 30-min exposure to iSMF after i.n. RWE challenge was able to reduce the airway inflammation. In addition, prolonged exposure to iSMF after RWE challenge decreased more effectively the severity of inflammation. In animals exposed to iSMF immediately after challenge, RWE induced a lower increase in the total antioxidant capacity of the airways suggesting that effects of iSMF on allergic inflammation were mediated at least partly by modulation of ROS levels. These data indicate that iSMF is able to reduce the inflammation in an in vivo system, despite the fact that it does not interfere with ROS-production of RWE NAD(P)H oxidases directly in cell free conditions.
Grants: TAMOP 4.2.2.A-11/1/KONV-2012-0023, Janos Bolyai Fellowship, Bridging Fund 2012
Keywords:
Oxidative Stress,
static magnetic field,
pollen allergy,
Allergic Airway Inflammation,
mouse model
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Csillag
A,
Kumar
BV,
Szabo
K,
Pazmandi
K,
Rajnavolgyi
E,
Laszlo
J and
Bacsi
A
(2013). Effect of static magnetic field on pollen-induced allergic airway inflammation in a murine model.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00255
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Received:
13 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Attila Bacsi, University of Debrecen, Department of Immunology, Debrecen, H-4032, Hungary, bacsi.attila@gmail.com