Genes affecting MMP expression are down-regulated in patients with COPD and bronchial asthma
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1
Institute of Molecular and Translational Medicine, Laboratory of Immunogenomics and Immunoproteomics, Czechia
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2
Palacky University, Czechia
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3
Faculty of Medicine and Dentistry, Palacky University, Dept. of Respiratory Medicine and Tuberculosis, Czechia
Proteolytic enzymes such as matrix metalloproteinases (MMPs) and others are up-regulated in chronic obstructive pulmonary disease (COPD) and bronchial asthma (BA). Their exceeding expression and proteolytic activity could result from insufficient inhibition and deregulated mRNA stability. We, therefore, investigated two inhibitors of MMPs (RECK and PTEN) and also RNA-binding proteins affecting mRNA stability (AUF1, HuR and PCBP2) in the both diseases. Real time-PCR method with PSMB2 as housekeeping gene was used to quantify the relative expression of RECK, PTEN, AUF1, HuR and PCBP2 in bronchoalveolar cells from 22 control subjects, 30 COPD patients and 19 patients with BA. The numbers of RECK (p=0.004, p=0.03)and AUF1 (p=0.01, p=0.02) mRNA transcripts were lower in both groups of COPD and BA patients than those in healthy control subjects. In comparison to controls, mRNA expression of HuR was decreased in COPD patients (p=0.03). There was a positive correlation between RECK expression and AUF1 (p<0.001), PCBP2 (p<0.001) and HuR (p=0.02), respectively. Overall, expression of the investigated genes did not differ between COPD patients and those with BA (p>0.05), smoking status was reflected in our analyses. In conclusion, the down-regulation of RECK observed in this study may contribute to protease/anti-protease imbalance in COPD and BA. As this has been the first investigation of RNA-binding proteins in these inflammatory diseases, further studies are needed to clarify the involvement of these molecules in COPD and/or BA pathomechanisms. Grant support IGA PU LF 2013_009 and CZ.1.05./2.1.00/01.0030
Keywords:
COPD,
Asthma,
RNA-Binding Proteins,
inhibitors of MMPs,
metalloproteinase
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Navratilova
Z,
Krsjakova
T,
Novosadova
E,
Zatloukal
J,
Kolek
V and
Petrek
M
(2013). Genes affecting MMP expression are down-regulated in patients with COPD and bronchial asthma.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00291
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Received:
14 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Zdenka Navratilova, Institute of Molecular and Translational Medicine, Laboratory of Immunogenomics and Immunoproteomics, Olomouc, Czechia, navratilz@yahoo.com