Pro-inflammatory cytokines analysis in HPV-positive cancer cells
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1
Sapienza University of Rome, Department of Medico-Surgical Sciences and Biotechnologies, Italy
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2
Istituto Superiore di Sanità, Department of Infectious, Parasitic and Immune-mediated Diseases, Italy
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3
Consiglio Nazionale delle Ricerche, Institute of Molecular Biology and Pathology, Italy
Recent data have expanded the concept that inflammation is a critical component of tumor progression. Many cancers arise from sites of infection, chronic irritation and inflammation. It is becoming clear that the tumor microenvironment is an indispensable participant in the neoplastic process, fostering proliferation, survival and migration. In addition, tumor cells have co-opted some of the signalling molecules of the innate immune system, such as selectins, chemokines and their receptors for invasion, migration and metastasis. Virus-induced tumors, like cervical high risk Papillomavirus-induced Squamous Cell Carcinomas (SCC), represent a paradigmatic example of the tight interplay between inflammatory responses and malignant transformation as inflammation is an integral part of the innate antiviral response.
To establish a tumorigenic role of inflammatory mediators in HPV-positive SCC, we analysed by real time RT-PCR the expression of inflammatory cytokines, chemokines and related molecules (i.e. IL-1α, IL-1β, IL-1RA, IL-1R1, IL-6, IL-8, MIP-1α and MIP-1β) in several HPV-positive carcinoma cell lines as well as in HPV-negative SCC (i.e. C33A). We also analysed human foreskin keratinocytes transduced by E6 and E7 from mucosal (HPV-16) or cutaneous (HPV-38) genotypes.
Our results indicate that in HPV-positive SCC the level of IL-1β mRNA is augmented compared to HPV-negative SCC. Interestingly, despite huge increase in mRNA levels, these cells didn’t secrete any IL-1β. On the other hand, IL-6 mRNA appears upregulated in the same cells as well as secreted in the supernatants. Finally, we also tested the effect of the antiviral cytokine IFN-β on the levels of these pro-inflammatory mediators.
Keywords:
Cancer,
Inflammation,
Human papillomavirus,
Beta interferon,
Squamous cell carcinoma
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Mangino
G,
Zangrillo
MS,
Chiantore
MV,
Iuliano
M,
Severa
M,
Fiorucci
G,
Coccia
EM and
Romeo
G
(2013). Pro-inflammatory cytokines analysis in HPV-positive cancer cells.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00306
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Received:
20 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Giorgio Mangino, Sapienza University of Rome, Department of Medico-Surgical Sciences and Biotechnologies, Latina, 04100, Italy, giorgio.mangino@uniroma1.it