Morphologic, flow cytometric, functional, and molecular analyses of S100B positive lymphocytes, unique cytotoxic lymphocytes containing S100B protein.
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1
Okayama University, Japan
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2
Kagawa Prefectural University of Health Sciences, Japan
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3
Kawasaki College of Allied Health Professions, Japan
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4
Okayama Red Cross Hospital, Japan
Little is known about the S100B+ lymphocytes, which are unique human peripheral blood lymphocytes (PBL) containing the S100B protein. It has recently been shown that S100B is released from various types of S100B+ cells and exhibits varied cytokine-like activities. In this study, we precisely characterized the S100B+ lymphocytes of healthy adults with respect to the proportion in the whole PBL, immunophenotypes, function, and their S100B mRNA expression and also evaluated their S100B-releasing activity upon stimulation. S100B+ lymphocytes were detected in all individuals examined, and the proportion of S100B+ lymphocytes in the whole PBL ranged from 0.42 to 16.15% (mean 4.21%). In addition, two subtypes of S100B+ lymphocytes, CTL subtype (CD3+ CD8+ CD16-) and NK subtype (CD3- CD8- CD16+) were detected. The majority of the CTL subtype of S100B+ lymphocytes expressed the -T cell receptor. Surprisingly, S100B mRNA was detected not only in S100B+ lymphocytes, but also in every S100B- lymphocytes, although the expression levels of S100B mRNA in S100B- lymphocytes were much lower than those of S100B+ lymphocytes. The CTL subtype of S100B+ lymphocytes exhibited blastic morphological changes, proliferated and released S100B upon stimulation with PHA. The NK subtype of S100B+ lymphocytes exhibited morphological NK activity when co-cultivated with NK-sensitive target, K-562 cells. Thus, the CTL subtype S100B+ lymphocytes exhibit the biological characteristics of T cells, while the NK subtype S100B+ lymphocytes exhibit the characteristics of NK cells. These results suggest that S100B+ lymphocytes are a particular subtype of cytotoxic lymphocytes that play a unique role in anti-tumor immunity.
Keywords:
S100B,
Peripheral Blood,
Cytotoxic T Cells,
NK cells,
Lymphocytes
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Miki
Y,
Gion
Y,
Mukae
Y,
Hayashi
A,
Sato
H,
Yoshino
T and
Takahashi
K
(2013). Morphologic, flow cytometric, functional, and molecular analyses of S100B positive lymphocytes, unique cytotoxic lymphocytes containing S100B protein..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00311
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Received:
15 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Kiyoshi Takahashi, Okayama University, Okayama, Japan, dend@md.okayama-u.ac.jp