PD-L1 expression induced by the 2009 pandemic influenza A(H1N1) virus impairs the human T cell response
Nuriban
Valero-Pacheco1, 2,
Luz María
Mora-Velandia2,
Lourdes
Arriaga-Pizano2,
Eduardo
Ferat-Osorio2,
Rodolfo
Pastelin-Palacios3,
Miguel Ángel
Villasís-Keever4,
Celia
M.
Alpuche-Aranda5,
Luvia
E.
Sánchez-Torres1,
Laura
C.
Bonifaz2,
Armando
Isibasi2 and
Constantino
López-Macías2*
-
1
National School of Biological Sciences, National Polytechnic Institute, Mexico City, Mexico, Mexico
-
2
Medical Research Unit on Immunochemistry (UIMIQ), Specialties Hospital, National Medical Centre “Siglo XXI,” Mexican Social Security Institute (IMSS), Mexico
-
3
Facultad de Química, Universidad Nacional Autónoma de México, Mexico
-
4
Unidad de Investigación en Epidemiología Clínica, Hospital de Pediatría, Centro Médico Nacional Siglo XXI. Instituto Mexicano del Seguro Social, Mexico
-
5
Instituto Nacional de Salud Pública, Mexico
PD-L1 expression plays a critical role in the impairment of T cell responses during chronic infections; nevertheless, the expression of PD-L1 on T cells during acute viral infections, particularly with the pandemic influenza virus (A(H1N1)pdm09), as well as the consequences of PD-L1 expression during infection, have not been explored. We found that A(H1N1)pdm09 induced PD-L1 expression on human dendritic cells (DCs) and T cells. PD-L1 expression impaired the T cell response against A(H1N1)pdm09 by slightly decreasing CD4+ T cell proliferation, promoting CD8+ T cell death and reducing cytokine production. Furthermore, we found increased PD-L1 expression on DCs and T cells from influenza-infected patients from the first and second pandemic waves in Mexico City. PD-L1 expression on CD8+ T cells correlated inversely with T cell proportions in patients infected with A(H1N1)pdm09 but not in those with influenza-like illness. Therefore, PD-L1 expression on DCs and T cells could be associated with an impaired T cell response during acute infection with A(H1N1)pdm09 virus.
Acknowledgements
The authors acknowledge funding provided by the Mexican Social Security Institute (IMSS) through the Fondo de Investigación en Salud projects as follows: FIS/IMSS/PROT/703 and FIS/IMSS/PROT/G11/954. Funding was also provided by Instituto de Ciencia y Tecnología del Distrito Federal, project number PICDSI09-248, warded to C. L-M and A.I. N. V.-P. and L.M. M.-V. received Ph.D. fellowships from the Mexican National Science and Technology Council (CONACYT) and from the IMSS.
Keywords:
T cell responses,
Pandemic 2009 H1N1,
PD-L1,
Influenza A virus,
pandemic H1N1 influenza
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Valero-Pacheco
N,
Mora-Velandia
L,
Arriaga-Pizano
L,
Ferat-Osorio
E,
Pastelin-Palacios
R,
Villasís-Keever
M,
Alpuche-Aranda
CM,
Sánchez-Torres
LE,
Bonifaz
LC,
Isibasi
A and
López-Macías
C
(2013). PD-L1 expression induced by the 2009 pandemic influenza A(H1N1) virus impairs the human T cell response.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00323
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Received:
24 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Constantino López-Macías, Medical Research Unit on Immunochemistry (UIMIQ), Specialties Hospital, National Medical Centre “Siglo XXI,” Mexican Social Security Institute (IMSS), Mexico City, Mexico, constantino.lopez135@gmail.com