Prostaglandin D2 in inflammatory arthritis and synovial myeloid dendritic cells as a source of hematopoietic prostaglandin D-synthase
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1
Royal Adelaide Hospital, Rheumatology unit, Australia
Prostaglandin (PG)D2 has been shown to be an active agent in the resolution of experimentally induced inflammation. This study was undertaken to determine the presence of PGD2 in chronic joint effusions and to explore the potential contributions of myeloid dendritic cells (mDC) and monocytes to the intra-articular synthesis of PGD2.
Synovial fluid (SF) was obtained from patients with inflammatory arthritis and knee effusions. PGD2 and PGE2 were detected in SF by ultrahigh performance-tandem mass spectrometry. Cellular fractions in SF were separated by density-gradient centrifugation and flow cytometry. The expression of hematopoietic prostaglandin D synthase (hPGDS) and PGE synthase (PGES) mRNA was determined by RT-PCR.
Both PGD2 and PGE2 were detected in blood and SF, with PGD2 being more abundant than PGE2 in SF. mRNA for hPGDS was more abundant in SF mDC than SF monocytes (p<0.01) or PB monocytes (p<0.001). SF PGD2 and hPGDS mRNA in SF mDC were inversely associated with serum C-reactive protein (p<0.01) and erythrocyte sedimentation rate (p<0.01) and a positive correlation was found between SF PGD2 and expression of hPGDS by SF mDC (p<0.02).
The findings suggest synovial mDC may be an important source of hPGDS and that systemic disease activity may be influenced by actions of PGD2 in RA and other arthropathies.
Keywords:
Prostaglandin D2,
Prostaglandin E2,
Dendritic Cells,
Inflammatory arthritis,
Synovial Fluid
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Moghaddami
M
(2013). Prostaglandin D2 in inflammatory arthritis and synovial myeloid dendritic cells as a source of hematopoietic prostaglandin D-synthase.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00343
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Received:
19 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Mahin Moghaddami, Royal Adelaide Hospital, Rheumatology unit, Adelaide, South Australia, Australia, mahin.moghaddami@health.sa.gov.au