Interleukin-10 gene polymorphisms (-1082 A/G, -819 C/T, -592 C/A) in primary Sjögren's syndrome
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1
Universidad de Guadalajara, Mexico
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2
Hospital General de Occidente, Servicio de Reumatología, Mexico
Introduction: Primary Sjögren´s syndrome (pSS) is an autoimmune disease of unknown etiology affecting primarily salivary and lacrimal exocrine glands. It is characterized by a progressive lymphocytic infiltration where B cells produce a great variety of antibodies, including those against ribonucleoproteins Ro and La. IL-10 plays an important role in pSS by promoting the differentiation of B cells inducing antibody production. Three polymorphic sites located at positions -1082 (A/G), -819 (C/T) and -592 (C/A) in the IL-10 promoter show a linkage disequilibrium and form haplotypes that may influence the IL-10 production and consequently an increased activity of the disease. Objective: To determine the frequency of haplotypes in the IL-10 promoter gene in patients with pSS. Methodology: The study included 113 Mexican mestizo patients with pSS, classified according to the American-European Consensus criteria, from the Department of Rheumatology (Hospital General de Occidente, SSJ). As control group, 154 clinically healthy subjects were included. The polymorphisms were genotyped by PCR/RFLP technique. Statistical analysis was carried out with the Stata 9.0 and EmHapFre software. Results: Polymorphisms were in Hardy–Weinberg equilibrium (-1082 A/G p=0.8377, -819C/T p= 0.1174, -592 C/A p=0.7953) and showed high linkage disequilibrium (100%, pc=1.42 x 10-34).The most frequent haplotypes were: ACC (41-61%), ATT (21.38%), GTA (14.86%), and GCC (11.18%). GTC haplotype was associated with a 4.35-fold less probability to develop pSS [OR; 95%CI: 0.23 (0.064-0.792) p= 0.01]. Conclusion: Our results suggest that carriers of the GTC haplotype have a protective status for the development of pSS.
Keywords:
primary Sjögren´s syndrome,
Antibodies,
IL-10 promoter,
Linkage Disequilibrium,
Haplotypes
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Oregon-Romero
E,
Vázquez-Villamar
M,
Salazar-Camarena
DC,
Treviño-Talavera
BA,
Orozco-Barocio
G,
Muñoz-Valle
JF,
Valle-Delgadillo
YM and
Palafox-Sánchez
CA
(2013). Interleukin-10 gene polymorphisms (-1082 A/G, -819 C/T, -592 C/A) in primary Sjögren's syndrome.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00346
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Received:
15 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Edith Oregon-Romero, Universidad de Guadalajara, Guadalajara, Mexico, oregon_edith@hotmail.com