Transcript levels of complement regulatory proteins (CD35 and CD59) in rheumatoid arthritis patients and their relationship with disease activity and prognosis
-
1
All India Institute of Medical Sciences (AIIMS), Biochemistry, India
-
2
All India Institute of Medical Sciences (AIIMS), Medicine, India
Objectives: Complement regulatory proteins (CRPs) protect the host from tissue injury mediated by activated complement system. In rheumatoid arthritis (RA) exaggerated complement activation is the key event, due to which CRPs CD35 and CD59 may influence the disease pathology and prognosis. Therefore, we aimed at elucidating the leukocyte-CD35 (L-CD35) and L-CD59 transcript levels and the relationship of these two CRPs with the disease activity of RA patients.
Methods: 66 controls and 45 RA patients were recruited for this study. Out of 45 RA patients, 11 patients volunteered for the longitudinal study. L-CD35 and L-CD59 transcript levels were determined by real-time PCR and correlated with Circulating immune complexes (CIC) levels and Disease Activity Score-28 (DAS28).
Results: Transcript levels of L-CR1 (p < 0.05) and L-CD59 (p < 0.01) were lowered in patients as compared to controls. In longitudinal study, L-CR1 transcript levels (p < 0.05) increased in patients which responded to treatment which correlated with lowered CIC levels (p < 0.05) and DAS28 scores (p < 0.05) at W24 as compared to W0. No significant difference was observed for L-CR1 transcript levels in non-responder group and for L-CD59 transcript levels in responder and non-responder group at W24 as compared to W0.
Conclusions: This is the first study to assess the L-CR1 and L-CD59 transcript levels in RA patients. The findings indicate an intimate relation of L-CD35 transcript levels with the disease activity and prognosis in RA patients, and thus suggest L-CD35 transcript as a potential disease marker in RA.
Keywords:
Rheumatoid arthritis,
complement regulatory proteins,
Circulating immune complexes,
prognosis,
disease marker
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Anand
D,
Kanjilal
M,
Kaur
S,
Kumar
U and
Das
N
(2013). Transcript levels of complement regulatory proteins (CD35 and CD59) in rheumatoid arthritis patients and their relationship with disease activity and prognosis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00379
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
22 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Nibhriti Das, All India Institute of Medical Sciences (AIIMS), Biochemistry, New Delhi, Delhi, 110029, India, nibhriti@gmail.com