Analysis of NK cell subsets in children with atopic dermatitis
-
1
Istanbul University, The Institute of Experimental Medicine (DETAE), Department of Immunology, Türkiye
-
2
Marmara University Medical Faculty, Division of Pediatric Allergy and Immunology, Türkiye
-
3
Istanbul University, Cerrahpasa Faculty of Medicine, Department of Pediatric Infections Disease, Türkiye
Atopic dermatitis (AD) is a frequent chronic inflammatory skin disease which is often complicated by recurrent microbial superinfections. Numerous studies demonstrated increased frequency of allergen-specific Th2 cells producing increased IL-4, IL-5 and IL-13 in the peripheral blood of AD patients, however little is known about the role of natural killer (NK) cells. In this study, NK1, NK2 and NK regulatory cytokine profiles, the expression of activatory receptors as well as the cytotoxic activity of NK cells in AD were investigated. The study group consists of children with AD (n=14, mean age=7 ± 3.8) and healthy subjects (n=14, mean age=10 ± 4). The patients were multisensitized to at least three aeroallergens and had high serum total IgE levels. Cytotoxic activity, expression of CD16brightCD56dim and CD16dimCD56bright NK cell subsets, NK cell activatory receptors and intracellular IL-4, IL-10 and IFN-g levels were determined by flow cytometry. In AD patients the percentages of CD3-CD16+CD56+, CD16brightCD56dim NK cell subsets and expression of activatory receptors of NKG2D cells were significantly decreased compared to healthy subjects (p=0.000, p=0.000 and p=0.04, respectively). Although, IL-10 secreting regulatory and IL-4 secreting NK2 cells were increased, IFN-g secreting NK1 cells and cytotoxic activity of NK cells were found to be decreased in AD patients (p=0.000, p=0.000, p=0.014 and p=0.016, respectively). Our results suggested that impaired NK cell functions such as decreased expression of CD16bright subset, NKG2D expression as well as cytotoxic activity, high secretion of IL-10 and IL-4 might play a role in the pathogenesis of AD.
Keywords:
Natural Killer cells,
Allergy and Immunology,
Cytokines,
Cytotoxicity,
NAtural Killer Cell subsets
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Deniz
G,
Aktas Cetin
E,
Akdeniz
N,
Baris
S,
Kosker
M,
Camcioglu
Y and
Barlan
I
(2013). Analysis of NK cell subsets in children with atopic dermatitis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00420
Copyright:
The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers.
They are made available through the Frontiers publishing platform as a service to conference organizers and presenters.
The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated.
Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed.
For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions.
Received:
12 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Gunnur Deniz, Istanbul University, The Institute of Experimental Medicine (DETAE), Department of Immunology, Istanbul, Türkiye, gdeniz@istanbul.edu.tr