Complement Regulatory Protein MCP; CD46 in Rheumatoid Arthritis
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1
All India Institute of Medical Sciences, Biochemistry, India
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2
All India Institute of Medical Sciences, Deptt. of Medicine, India
Introduction: Rheumatoid Arthritis (RA) is a chronic inflammatory disease characterized by pain, swelling and progressive destruction of multiple joints, affecting approximately 1% of the adult human population. The growing importance of Membrane cofactor protein (MCP; CD46) not only as central membrane bound complement inhibitor but also as critical regulator of T-cell immunity, suggests that regulation of its expression may have significant effects on health and disease.
Objectives: Studies on animal models suggest disease modulating activity of Leukocyte CD 46 (L-CD46) in autoimmune disorders. We conducted a case-control study to explore the role of L-CD 46 in human RA.
Methodology: The L-CD46 expression in 40 healthy controls and 40 RA patients on different leukocyte subpopulations was observed by Flow cytometry and expression at mRNA level was monitored using Real-Time PCR. The clinical parameters Circulating Immune Complexes (CIC), Disease activity scores (DAS28), swollen and tender joint counts (SJC and TJC) were determined and correlated with L-CD46 expression in patients.
Results: In patients, the L-CD 46 expression reduced significantly on lymphocytes followed by monocytes. Further L-CD 46 transcripts declined significantly in patients. Significant inverse correlations of L-CD 46 expression on lymphocyte and monocyte surface and transcript level with DAS28, SJC and CIC were observed in patients.
Conclusions: Decline in the L-CD 46 levels suggests failure in protective role of L-CD 46 against complement mediated damage in RA patients. The negative correlations of L-CD 46 with disease activity indices suggest its significant association with the disease state. Follow-up study confirming L-CD 46 role as pivotal biomarker is warranted.
Keywords:
Rheumatoid arthritis,
MCP,
CD46,
complement,
Inflammation
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Pahwa
R,
Kumar
U and
Das
N
(2013). Complement Regulatory Protein MCP; CD46 in Rheumatoid Arthritis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00423
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Received:
09 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Nibhriti Das, All India Institute of Medical Sciences, Biochemistry, New Delhi, India, nibhriti@gmail.com