Knee injury with concomitant osteochondral fracture is associated with increased synovial fluid concentrations of SPARC, IL-8 and TNF-α.
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1
Department of Orthopaedics, Lund University, BMC C12, SE-221 84 Lund, Sweden., Sweden
Purpose: Approximately 50% of patients with an anterior cruciate ligament tear also sustain an osteochondral (OC) fracture in association with the injury. The aim of the present study was to investigate if concentrations of cartilage and bone markers and pro-inflammatory cytokines in synovial fluid (SF) collected from acutely injured knees were different between acutely injured knees with/without an OC fracture.
Methods: Synovial fluid with hemarthrosis was aspirated from acutely injured knees of 111 individuals (mean age 27 years, range 13-64 years, 22% women) in median 1 (range 0-23) day after the injury. Concentrations of sulphated glycosaminoglycan, cartilage ARGS, COMP osteocalcin, secreted protein acidic and rich in cysteine (SPARC), osteopontin and pro-inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) were analyzed. Ninety-eight of the 111 patients underwent MRI (1.5 Tesla) of the injured knee in median 8 (range 1-38) days after the injury.
Results: Thirty-eight of the acutely knee injured patients had an OC fracture with discontinuity of the cortical bone and 29 had an OC fracture without discontinuity of the cortical bone in the knee joint. Thirty-one patients did not sustain an OC fracture. Knees with an OC fracture with discontinuity of the cortical bone had significantly higher SF concentrations of SPARC (p = 0.034), IL-8 (p = 0.030) and TNF-α (p=0.005) compared to knees without an OC fracture after adjusting for days between injury and SF aspiration, age, sex and type of knee injury.
Conclusions: An associated OC fracture may cause a higher degree of joint inflammation after knee injury.
Keywords:
knee injury,
Anterior Cruciate Ligament,
osteochondral fracture,
MRI imaging,
biomarkers,
Cartilage,
Bone Marrow,
Fractures, Bone,
Inflammation Mediators
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Swärd
P,
Struglics
A,
Englund
M,
Roos
H and
Frobell
R
(2013). Knee injury with concomitant osteochondral fracture is associated with increased synovial fluid concentrations of SPARC, IL-8 and TNF-α..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00424
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Received:
27 Mar 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mr. Per Swärd, Department of Orthopaedics, Lund University, BMC C12, SE-221 84 Lund, Sweden., Lund, Sweden, pellesward@hotmail.com