Endocytosis and recycling of immune complexes by follicular dendritic cells enhances B cell binding and activation
Balthasar
A.
Heesters1, 2, 3*,
Priyadarshini
Chatterjee1, 2,
Young-A
Kim1, 2,
Santiago
F.
Gonzalez1,
Michael
P.
Kuligowski1,
Tomas
Kirchhausen2 and
Michael
C.
Carroll1, 2
-
1
Boston Children's Hospital, Program in Cellular and Molecular Medicine, United States
-
2
Harvard Medical School, United States
-
3
Utrecht University, Medical Microbiology, Netherlands
Follicular dendritic cells, which are stromal derived, are a major reservoir for antigen that is essential for formation of germinal centers, the site where memory and effector B cells differentiate. A long-standing question is how the follicular dendritic cells retain antigen in its native form for extensive periods and how they display it to specific B cells. We find that they take-up complement-coated immune complexes from non-cognate B cells via complement receptors (CD21/CD35) and rapidly internalize them by an actin dependent pathway. Immune complexes are retained intact within a cycling compartment and are displayed periodically on the cell surface where they are accessible to antigen- specific B cells. Thus, internalization of immune complexes into a non- degradative compartment and periodic cycling to the cell surface would explain how antigens are retained for long periods and made available to B cells.
Highlights:
-Visualize direct transfer of immune complexes from non-cognate B cells to FDC in vivo using multi-photon intravital imaging
-Uptake of immune complexes from non-cognate B cells by FDC is actin- dependent
-FDC rapidly internalize intact immune complexes into a cycling compartment -Immune complexes cycling to the FDC surface are displayed for B cell acquisition
- In vivo, FDC retain immune complexes in a cycling compartment for at least 16 days
Keywords:
Follicular Dendritic Cell (FDC),
Cycling,
immune complexes,
B cells,
Complement System Proteins,
Complement Receptor 2(CR2)
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Heesters
BA,
Chatterjee
P,
Kim
Y,
Gonzalez
SF,
Kuligowski
MP,
Kirchhausen
T and
Carroll
MC
(2013). Endocytosis and recycling of immune complexes by follicular dendritic cells enhances B cell binding and activation.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00438
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Received:
12 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Mr. Balthasar A Heesters, Boston Children's Hospital, Program in Cellular and Molecular Medicine, Boston, United States, Balthasar.Heesters@childrens.harvard.edu