Acute inflammation loci influence tissue repair in mice
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1
Instituto Butantan, Brazil
AIRmax (high inflammation) and AIRmin (low inflammation) mouse lines homozygous for Slc11a1 S alleles, produced by genotype-assisted mating, differ in ability to completely repair ear holes. AIRmaxSS mice showed fast ear tissue regeneration while AIRminSS mice did not show regeneration after ear punch. In this work, two-millimeter ear holes were done in these mice for the characterization of inflammatory reaction. The local inflammatory response was more intense in AIRminSS than AIRmaxSS mice 24 and 48h after ear punch, which was demonstrated by histomorphometric analysis, multiplex assay for inflammatory cytokines such as IL-1β, IL-6 and MIP-2 and higher levels of MPO. Global gene expression analysis demonstrated distinct over-represented biological themes between AIRmaxSS and AIRminSS control mice. At 24h after punch, both AIRmaxSS and AIRminSS showed significant up-regulated genes related to inflammation. However, angiogenesis, epidermis development and collagen catabolic process were expressed only in AIRminSS. All down-regulated genes in response to wounding in AIRmaxSS were represented to muscle contraction which is known to be involved in healing with scarring. Microarray results were validated by qPCR. These results suggest that the degree of inflammatory response in the early events after injury drives tissue regeneration or wound healing after ear punch
Acknowledgements
Finantial Support: The State of São Paulo Research Foudantion (FAPESP) and National Council of Research (CNPq), Brasil.
Keywords:
mice genetically selected,
tissue repair,
Tissue Regeneration,
Gene Expression,
Inflammation
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Canhamero
T,
Corrêa
MA,
Fernandes
JG,
Borrego
A,
Jensen
JR,
Cabrera
WH,
Ribeiro
OG,
Starobinas
N,
Ibañez
OM and
De Franco
M
(2013). Acute inflammation loci influence tissue repair in mice.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00535
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Received:
22 Apr 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Marcelo De Franco, Instituto Butantan, Sao Paulo, Brazil, mdfranco@butantan.gov.br