TARGETING THE Plasmodium chabaudi MSP-1(19) PROTEIN TO THE DEC205 DENDRITIC CELL POPULATION INDUCES FASTER PARASITE CLEARANCE
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1
Universidade de Sao Paulo, Brazil
Malaria is an infectious disease distributed worldwide. It’s responsible for thousands of deaths every year, affecting mainly children. The acquired immune response during infection is partial and transitory. A membrane antigen of the merozoite is widely used as a vaccine target because of its immunogenic properties. This protein is expressed during schizogony as a precursor of 200kDa that undergoes successive cleavages, finally remaining anchored in the membrane as a portion of 19kDa named MSP-1(19), which has an important role in the reinvasion of the erythrocytes. Several studies have focused on the development of a vaccine against the parasite that leads to the maintenance of a protective memory response to different immunization schedules. A promising vaccine strategy is to target antigens directly to dendritic cells (DC) in vivo. This is accomplished by the administration of a monoclonal antibody that recognizes the endocytic receptor DEC205 present at the surface of DCs fused with an antigen of interest. Here we produced a hybrid αDEC205 coupled to the MSP-1(19) from Plasmodium chabaudi (AS) and used it to prime C57BL/6 mice that were subsequently boosted with the recombinant MSP-1(19) protein in the presence of Poly (I:C). Anti P. chabaudi MSP-1(19) antibodies were only detected in mice that received a boost with the recombinant MSP-1(19) protein. Protection against acute infection correlated with higher antibody titers. Adoptive transfer of blood from immunized and challenged mice showed a faster parasite clearance when MSP-1(19) was targeted to dendritic cells using the αDEC-MSP-1(19) antibody.
Acknowledgements
Supported by FAPESP, CNPq and INCTV.
Keywords:
Malaria,
MSP1,
Dendritic Cells,
DEC205,
clearence
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Panatieri
RH,
Da Silva
HB,
Yamamoto
MM,
Lima
MD and
Boscardin
SB
(2013). TARGETING THE Plasmodium chabaudi MSP-1(19) PROTEIN TO THE DEC205 DENDRITIC CELL POPULATION INDUCES FASTER PARASITE CLEARANCE.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00555
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Received:
17 May 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Miss. Raquel H Panatieri, Universidade de Sao Paulo, Sao Paulo, Brazil, rachhpp@yahoo.com.br