Immune cells functional disorder in case of preeclampsia
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1
Scientific research institute of Obstetrics and Gynaecology named after D.O.Ott Russian Academy of Medical Science, St.Petersburg, Russian Federation, Russia
Preeclampsia is associated with functional disorder of immune cells that can affect their phenotype and influence their functions.
The aim was to assess expression of surface receptors on lymphocytes, NK- and NKT-cells, monocytes and its adhesion to endothelium.
Peripheral blood was obtained from 75 healthy non-pregnant women, 69 healthy pregnant women (38-39 weeks) and 79 pregnant women with preeclampsia (38-39 weeks). Peripheral blood mononuclear cells (PBMC) were stained with antibodies to CD3, CD4, CD8, CD16, CD56, CD14, CD11a, CD11b, CD11c, СD18, CD29, CD49d, CD31, CD44, CD47, CD54, CD58, CD62L, integrin β7, HLA-DR, CD119 and analyzed by FACSCantoII.
The adhesion of monocytes and lymphocytes to the intact and TNFα activated endothelial cells increased in preeclampsia as compared with physiological pregnancy. The amount of CD8+ T-lymphocytes and CD4+ T-lymphocytes expressing CD29, CD49d and CD54 decreased in case of preeclampsia comparing with healthy pregnant women. There was also a 6,5-fold increase of CD18 expression intensity by CD8+ T-lymphocytes. In case of uncomplicated pregnancy and in case of pre–eclampsia the amount of NK–cells expressing CD29 and CD49d was elevated. At preeclampsia the amount of NK–cells expressing CD11c, integrin β7 and CD47 and also expression of CD31 and CD11c by NKT-cells increased. The amount of monocytes expressing adhesion molecules didn`t differ between physiological pregnancy and preeclampsia. But the amount of CD119+ monocytes and their CD119-expression intensity were elevated in case of preeclampsia.
These results indicate that preeclampsia is associated with phenotypical changes of PBMC that facilitate their adhesion to uterine vessel endothelium.
Keywords:
Preeclampsia,
Lymphocytes,
NK-cells,
Monocytes,
Endothelium
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Sokolov
D,
Onohina
Y,
Mikhaylova
V,
Ovchinnikova
O,
Korenkov
D and
Selkov
S
(2013). Immune cells functional disorder in case of preeclampsia.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00782
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Received:
13 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Dmitry Sokolov, Scientific research institute of Obstetrics and Gynaecology named after D.O.Ott Russian Academy of Medical Science, St.Petersburg, Russian Federation, St.Petersburg, 199034, Russia, falcojugger@yandex.ru