Anti-DEC-205 receptor carrying P10 induces protective response in experimental Paracoccidioidomycosis
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1
University of São Paulo, Brazil
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2
Unifesp, Brazil
Paracoccidioidomycosis is a systemic mycosis caused by "Paracoccidioides brasiliensis", a thermally dimorphic fungus. It occurs in Latin America and is the most frequent endemic systemic mycosis in many countries of South America, where almost 10 million people are believed to be infected. In Brazil, it is ranked as the tenth cause of death among chronic infectious and parasitic diseases. It is likely that vaccines will need to elicit strong T cell-mediated immunity composed of IFNγ secreting CD4+ helper and CD8+ cytolytic T lymphocytes. To induce such responses, it would be valuable to harness the dendritic cell (DC) system of antigen-presenting cells. To assess the potential of targeting P10, a peptide derived from gp43 secreted by the fungus, directly to DCs, we cloned its sequence in fusion with a monoclonal antibody to the DEC-205 receptor, an endocytic receptor abundant on these cells in lymphoid tissues. We verified that αDEC/P10 antibody response induced large IFNγ-producing cells after single dose administration in relation to P10, even being administered at a lower concentration. However, this response was not maintained after the second dose of the antibody. After challenge of animals immunized with two doses of the antibody, we detected high levels IFNγ and IL-4 in the lung tissue was statistically higher in αDEC/P10 and ISO/P10 compared to administration of P10 and control Poly I:C. The results obtained indicate that targeting of P10 through αDEC/P10, in the presence of Poly I:C, is a promising strategy for vaccination against "P. brasiliensis".
Acknowledgements
Supported: FAPESP and CNPq.
Keywords:
"Paracoccidioides brasiliensis",
Dendritic Cells,
DEC205,
Vaccination,
Immunotherapy
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Dos Santos
SS,
Rampazo
EV,
Ferreira
KS,
Taborda
CP,
Boscardin
SB and
Almeida
SR
(2013). Anti-DEC-205 receptor carrying P10 induces protective response in experimental Paracoccidioidomycosis.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00815
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Received:
18 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Suelen S Dos Santos, University of São Paulo, São Paulo, Brazil, suelen.silvana.santos@gmail.com