CD49b regulates the establishment and maintenance of T helper cell memory
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1
German Rheumatism Research Center Berlin, Germany
We have demonstrated that professional memory CD4 T cells reside and rest in the bone marrow (BM) adhering to IL-7-expressing stromal niches. Their absence induces a defective generation of high-affinity long-lived plasma cells. However, the molecular mechanisms on their establishment and maintenance remain unclear. We here demonstrate the roles of CD49b, which is also called integrin alpha2 and is expressed on most memory CD4 T cells, in the establishment and maintenance of memory CD4 T cells in the BM. We first counted antigen-specific effector and memory CD4 T cells in immunized CD49b-deficient and wild-type mice. The number of CD49b-deficient memory CD4 T cells was significantly impaired compared to wild-type cells, while the number of effector cells in both mice was comparable. So, to clarify why CD49b-deficient memory CD4 T cells are not generated, we transferred CD49b-deficient effector CD4 T cells into naive mice and evaluated their migration ability into the BM. The loss of CD49b induced a defective migration of effector cells into the BM. Then, to examine a role of CD49b in the maintenance of memory CD4 T cells, we inhibited the integrin during memory phase with injection of Fab fragments of anti-CD49b neutralization antibodies. The inhibition of CD49b reduced the number of memory CD4 T cells in the BM. We suggest that CD49b regulates the establishment and maintenance of memory CD4 T cells controlling the immigration of memory cell precursors into the BM and also the persistence of memory CD4 T cells on their survival niches.
Keywords:
T helper cell memory,
CD49b,
Collagen,
niche,
Immunological memory,
Bone Marrow
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Hanazawa
A and
Tokoyoda
K
(2013). CD49b regulates the establishment and maintenance of T helper cell memory.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00827
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Received:
21 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Asami Hanazawa, German Rheumatism Research Center Berlin, Berlin, Germany, ahanazawa@ciea.or.jp
Dr. Koji Tokoyoda, German Rheumatism Research Center Berlin, Berlin, Germany, tokoyoda@tottori-u.ac.jp