Characterization of antibody responses to M13 phage vaccine: MyD88-dependent and T-cell independent IgG subclass responses.
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1
Department of Chemistry and Biotechnology, Graduate School of Science and Engineering, Kagoshima University, Japan
M13 phage can be administered in PBS in mice to evoke a strong primary IgG response that is dependent on MyD88 signaling (Ref.1). Even a single immunization with 1011 pfu of phage induced a long-lasting antibody response.
M13 phage injected by the intraperitoneal and subcutaneous routes induced serum IgG subclass responses to M13 phage in C57BL/6 mice dominated by IgG2c, IgG2b and IgG3 between 1 and 2 weeks of post injection. After a secondary injection of M13 phage, all four subclasses including IgG1 were detected at the same level. The same IgG subclass profiles was observed in athymic (nu/nu) BALB/c mice except the Ab level which was at least 20-fold lower than that observed in BALBc and C57BL/6 mice, indicating T-cell independent IgG class swithing. We have preiviously reported that phage-specific IgG2c, IgG2b and IgG3 responses in TLR2-, TLR4-, TLR7- or TLR9-deficient mice, except the primary IgG1 level was significantly elevated only in TLR9-deficient mice. These data suggested that the IgG1 producing cells in the secondary response in wt mice was derived from a distinct B-cell repertoire or subpopulation involved in the primary response, and IgG1 response to M13 phage is regulated by TLR9. We are currently analyzing which sensor molecules of innate immunity are involved in the recognition of M13 phage. The ability of M13 phage to induce a strong IgG response suggests a possible application of peptide-displaying phage clones as vaccine carriers for induction of therapeutic antibodies.
References
1. Hashiguchi S, Yamaguchi Y, Takeuchi O, Akira S, Sugimura K. Immunological basis of M13 phage vaccine: Regulation under MyD88 and TLR9 signaling. Biochem. Biophys. Res. Commun. (2010) 402: 19-22.
Keywords:
Bacteriophage M13,
Vaccines,
MyD88,
T cell independent,
IgG subclasses
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Innate immunity
Citation:
Hashiguchi
S and
Sugimura
K
(2013). Characterization of antibody responses to M13 phage vaccine: MyD88-dependent and T-cell independent IgG subclass responses..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00829
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Received:
21 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Prof. Kazuhisa Sugimura, Department of Chemistry and Biotechnology, Graduate School of Science and Engineering, Kagoshima University, Kagoshima, Japan, kazu@be.kagoshima-u.ac.jp