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Peptide Microarrays with Near Zero background enable high quality and massive data production from seroscreening of autoimmune diseases

Mo Huang1, 2, 3, Yudong Lu1, 2, 3, Cen Liu1, 2, 3, Zhong Li1, Xing Liu1 and Hongwei Ma1*
  • 1 Suzhou Institute of Nano-Tech and Nano-Bionics(SINANO),Chinese Academy of Sciences, China
  • 2 Institute of Biophysics, Chinese Academy of Sciences, China
  • 3 Graduate University of the Chinese Academy of Sciences, China

For the ability of simultaneous analysis of thousands of substances, protein/peptide microarray technology has emerged as an important tool in many fields including disease diagnosis,protemics, and drug discovery. But the high background intensity due to nonspecific protein adsorption has made the data extraction and analysis of protein/peptide microarray more complicated, and then hindered application of the technology in high throughput screening. Here we show the peptide microarray based on iPDMS, an advanced biomaterial which has the intrinsic nonfouling property, can maintain near zero background, which enable high quality and massive data production from seroscreening.

In our previous study, we found the substrate iPDMS showed excellent nonfouling property in sandwich ELISA for tumor marker detection. Here in the seroscreening by peptide microarray based on iPDMS, a maximal [IgG] of 0.5 mg mL-1 was determined to achieve near zero background without any blocking procedure. Since typical [IgG] in human serum is 10-14 mg mL-1, a minimal of 20 fold serum dilution is required. With this near zero background performance, peptide microarray has been successfully applied in epitope identification of autoimmune diseases, such as type 1 diabetes mellitus, systemic lupus erythematosus, and autoimmune thyroid disease. And we believe that peptide microarray based on iPDMS has enormous potentiality in epitope identification and clinical diagnosis of autoimmune diseases.

Acknowledgements

Supported by CAS KJZD-EW-J01

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Keywords: peptide microarray, anti nonspecific protein adsorption, Autoimmune Diseases, Epitope Mapping, seroscreening

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Immune-mediated disease pathogenesis

Citation: Huang M, Lu Y, Liu C, Li Z, Liu X and Ma H (2013). Peptide Microarrays with Near Zero background enable high quality and massive data production from seroscreening of autoimmune diseases. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00831

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Received: 20 Jun 2013; Published Online: 22 Aug 2013.

* Correspondence: Prof. Hongwei Ma, Suzhou Institute of Nano-Tech and Nano-Bionics(SINANO),Chinese Academy of Sciences, Suzhou, China, hwma2008@sinano.ac.cn