Study the polymorphism of lcr1 gene in different Leishmania species
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1
Pasteur Institute of Iran, Immunology, Iran
Introduction
Leishmania parasites are the causative agent of a wide range of diseases in human and other mammals. There is no efficient preventive measure for these diseases and their therapy remains difficult with many side effects. Hence, search for a vaccine for this disease is important. Lcr1 is an immunogenic gene discovered in Leishmania infantum chagasi that causes immunologic response in human, so its molecular identification will be very important. The aim of this research is to study the DNA sequence of lcr1 gene by molecular methods in some species of Leishmaina parasites.
Materials and Methods
Two Leishmania infantum isolates (strains MHOM.IR.04.IPI-UN10 and MCAN/IR/2010/Meshkinshahr) and one Leishmania tropica isolate (strain MHOM.AF.88.KK27) were cultured in NNN media and genomic DNA were extracted. Parasite species were verified as by sequencing of ITS1 gene. Lcr1 gene was amplified by lcr1 specific primers in all the strains. The PCR products were electrophoresed and their restriction fragment patterns were defined. All PCR products were sequenced at least twice by forward and reverse primers specific for lcr1. The DNA sequences were analyzed by softwares and the correct sequence were determined.
Conclusion
Our results show that lcr1 gene is completely conserved between the two strains of Leishmania infantum and it is nearly conserved in Leishmania tropica. Conservation of this immunogenic gene (lcr1) adds to its value as an immunogenic molecule with potentials in prevention, diagnosis, and treatment of leishmaniasis.
Support: This research received financial supports from Pasteur Institute of Iran (Research Project No. 314).
Keywords:
Leishmania infantum,
Laishmania tropica,
immunogenic molecule,
Polymorphism, Genetic,
DNA sequence
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Niknam
HM,
Abrishami
F,
Fatemi
K and
Azimian
H
(2013). Study the polymorphism of lcr1 gene in different Leishmania species.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00873
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Received:
20 Jun 2013;
Published Online:
22 Aug 2013.
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Correspondence:
Dr. Hamid M Niknam, Pasteur Institute of Iran, Immunology, Tehran, 13164, Iran, mahmoudzadehh@yahoo.com