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Modulation of Moraxella catarrhalis and nontypeable Haemophilus influenzae phagocytosis by neuropeptides NPY and CGRP

Daria Augustyniak1*, Judyta Nowak1 and Adam Jankowski2
  • 1 University of Wroclaw, Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, Poland
  • 2 Department and Clinics of Pediatrics, Immunology and Rheumatology of Developmental Age, Medical University of Wroclaw, Poland

Introduction: Colonization of respiratory tract is the first step in the infectious process. The direct and indirect antimicrobial action of many neuropeptides has been confirmed [1-4]. Since mucosal surfaces of the upper respiratory tract are richly innervated, human-restricted respiratory pathogens such as Moraxella catarrhalis and nontypeable Haemophilus influenzae are potentially exposed to the action of released neuropeptides including neuropeptide Y (NPY), and calcitonin-gene related peptide (CGRP).
The study was designed to verify neuropeptide-mediated modulation of human neutrophils (PMNs) phagocytosis against studied bacteria by determination of two critical phagocytic steps namely bacterial uptake and respiratory burst response [4].

Methods: The phagocytic uptake of antibody-opsonized and non-opsonized FITC-labeled bacteria was measured using FACs. Reactive oxygen species (ROS) formation by phagocytosing PMNs was measured using luminol-dependent chemiluminescence assay (CL).

Results: We found the bimodal modulatory action of neuropeptides that was observed in distinct types of pathogen recognition (opsonic versus nonopsonic). The phagocytic uptake of both opsonized mucosal pathogens by PMNs was markedly improved in the presence of NPs. The uptake remained unchanged in the presence of nonopsonized bacteria. In the case of ROS production only CGRP significantly intensified the respiratory burst of PMNs triggered by phagocytosable nonopsonic M. catarrhalis. The observed stimulating effect of CGRP was not preserved for opsonized bacteria. The contradictory action of NPs on engulfment and ROS response seems to be determined both by the nature of pathogen recognition and the physiological status of PMN donor.

Conclusion: The results suggest that physiologic concentrations of CGRP and NPY are modulators of PMNs uptake and respiratory burst in response to M.catarrhalis and H.influenzae and may be valuable innate components of immunity against these pathogens.

References

1. Bedoui, S., Kawamura, N., Straub, R.H., Pabst, R., Yamamura, T., von Horsten, S. (2003). Relevance of neuropeptide Y fo the neuroimmune crosstalk. J. Neuroimmunol. 134, 1–11
2. Brogden, K.A, Guthmiller, J.M., Salzet, M., Zasloff, M. (2005). The nervous system and innate immunity: the neuropeptide connection. Nat. Immunol. 6, 558–563.
3. Augustyniak, D., Nowak, J., Lundy, F.T. (2012). Direct and indirect antimicrobial activities of neuropeptides and their therapeutic potential. Curr. Protein Pept. Sci. 13, 723-38.
4. Augustyniak, D,, Jankowski, A., Mackiewicz, P., Skowyra, A., Gutowicz, J., Drulis-Kawa, Z. (2012). Innate immune properties of selected human neuropeptides against Moraxella catarrhalis and nontypeable Haemophilus influenzae. BMC Immunol. 2,13-24.

Keywords: Phagocytosis, Neuropeptides, NPY, CGRP, Neutrophils, Moraxella catarrhalis, Haemophilus influenzae

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Innate immunity

Citation: Augustyniak D, Nowak J and Jankowski A (2013). Modulation of Moraxella catarrhalis and nontypeable Haemophilus influenzae phagocytosis by neuropeptides NPY and CGRP. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00909

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Received: 28 Jun 2013; Published Online: 22 Aug 2013.

* Correspondence: Dr. Daria Augustyniak, University of Wroclaw, Department of Pathogen Biology and Immunology, Institute of Genetics and Microbiology, Wroclaw, Poland, daria.augustyniak@microb.uni.wroc.pl