Serum Amyloid A impacts the growth of melanoma cells and may be a link between inflammation and cancer outcome.
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1
University of Sao Paulo, Brazil
Serum amyloid A (SAA), an inflammatory protein, has been called attention due its increased concentrations in plasma of cancer patients. Recently, it was suggested an immune modulation mediated by SAA in melanoma patients (Nat Immunol., 11:1039-46, 2010). Previous study from our group showed increased proliferation of human glioma lines in the presence of SAA. Furthermore SAA also affected migration and invasion (Mediators of Inflammation, 2013, in press). To verify if these findings could be extended to other tumor we evaluate the effects of SAA (5 or 10 µg/mL) on proliferation, migration, invasion and cytokine release on melanoma cells (SK-Mel 19, 28, 103 and 147). We used [3H]-thymidine incorporation and clonogenic assay to evaluate proliferation, flow cytometry for necrosis and apoptosis, scratch test and Boyden chamber assay to evaluate mobility and ELISA for cytokine release. We observed that SAA inhibited proliferation without inducing cells death, however, by optical microscopy, we observed that SAA could cause morphologic alterations similar to cell senescence. Some of the cell lines seemed to be more sensitive to SAA in relation to cellular motility. In these cases it was observed decreased migration and/or invasion. Furthermore, SAA induced the release of IL-6 and IL-8 cytokines on melanoma cells. Our finding demonstrated that SAA might, in some cases, impact melanoma growth and emphasizes the possibility to be one of the factors that links inflammation with cancer outcome.
Acknowledgements
This work was supported by Grants from support: Sao Paulo Research Foundation (FAPESP, Sao Paulo); National Counsel of Technological and Scientific Development (CNPq, Brasília); and Coordination of Improvement of Higher Education Personnel (CAPES, Brasília)
References
De Santo, C., Arscott, R., Booth, S., Karydis, I., Jones, M., Asher, R., Salio, M., Middleton, M., Cerundolo, V. (2010). Invariant NKT cells modulate the suppressive activity of IL-10-secreting neutrophils differentiated with serum amyloid A. Nat Immunol..11, 1039-46.
Knebel, F.H., Albuquerque, R.C., Massaro, R.R., Maria-Engler, S.S., Campa, A. (2013). Dual effect of serum amyloid A on the invasiveness of glioma cells. Mediators of inflammation. 2013.
Keywords:
Melanoma,
Serum Amyloid A Protein,
proliferation,
Inflammation,
invasiveness
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune receptors and signaling
Citation:
Bellé
LP,
Knebel
FH,
Albuquerque
R,
Maria-Engler
SS and
Campa
A
(2013). Serum Amyloid A impacts the growth of melanoma cells and may be a link between inflammation and cancer outcome..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00912
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Received:
26 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Ms. Luziane P Bellé, University of Sao Paulo, São Paulo, Brazil, luzibelle@usp.br