Quality controls of Cell-Based therapies: Assessment of conditioned Dendritic Cells phenotypes by Global Gene Expression Profiles
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1
University of Milano-Bicocca, Department of Surgery and Interdisciplinary Medicine, Italy
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2
Genopolis, Department of Biotechnology and Bioscience, Italy
Whole-cell immunotherapies have shown promising results in clinical trials. Nevertheless, the complex nature of the cellular product and the individual variability associated to their preparation makes cellular immunotherapies less well characterized. Therefore, one major challenge in the development of whole cell therapies is the ability to demonstrate a clear comparability of the cellular product obtained after the application of different differentiation settings and conditioning protocols in the manufacturing process.
Research in our lab is focused towards the development of novel molecular assays that can be used as quality controls in cell-based therapies. Using a functional genomics approach we have been able to select a genetic signature of activation of DCs in vitro. We selected lists composed of 44 genes able to discriminate different DCs phenotypes. We validated the signature in vivo in DCs derived from a mouse model of inflammation. The selected signature has been further validated in human derived DCs in order to develop a quality control molecular assay for DCs-based therapies. Therefore, the basic methods to select a genetic signature of DCs that may predict in vivo functional activity and its applications in cellular immunotherapies are presented.
Acknowledgements
Italian Foundation for Multiple Sclerosis. FISM2009
Italian Ministry of University and Scientific Research, PRIN2009
7FP Project: FIGHT-MG 242210.
7FP Project: TOLERAGE HEALTH-F4-2008-202156.
Keywords:
Dendritic Cells,
Immunotherapy,
Gene Expression Profiling,
Cell based therapies,
Immune Regulation
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Baldassarri
S,
Ripamonti
C,
Fumagalli
S and
Foti
M
(2013). Quality controls of Cell-Based therapies: Assessment of conditioned Dendritic Cells phenotypes by Global Gene Expression Profiles.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.00915
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Received:
28 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Maria Foti, University of Milano-Bicocca, Department of Surgery and Interdisciplinary Medicine, Milan, Italy, maria.foti@unimib.it