Characterization of IL-17 and IL-17 receptor families in lampreys
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1
Emory University, Emory Vaccine Center and Department of Pathology and Laboratory Medicine, United States
Interleukin-17 family members have been identified in lampreys. These jawless vertebrates have an alternative adaptive immune system wherein variable lymphocyte receptors (VLRs) composed of leucine-rich-repeats are used for antigen recognition. Nevertheless, jawless vertebrates have T- and B-like lymphocytes that express VLRA and VLRB respectively. Prior studies in our laboratory suggested that IL-17 transcripts (IL-17D orthologue) are expressed preferentially by VLRA+ lymphocytes and at higher levels after activation. Conversely, VLRB+ lymphocytes express IL-17 receptor transcripts (IL-17RA orthologue named IL-17RA1). Because lampreys represent an early phylogenetic juncture between innate and adaptive immunity, we sought to characterize the IL-17 family members and their cognate receptors in order to examine their potential roles in coordinating VLRA+ and VLRB+ cellular interactions. Nested PCR was used to obtain full-length cDNAs of the lamprey IL-17D (591-bp open reading frame) and three IL-17R family members with IL-17RA homology (IL-17RA1, IL-17RA2 and IL-17RA3). Quantitative RT-PCR analysis indicated that the lamprey IL-17D orthologue is expressed highly in skin, gill and VLRA+ lymphocytes, while lamprey IL-17RA1 is expressed highly in hematopoietic tissue typhlosole, VLRB+ lymphocytes and monocytes. A recombinant IL-17D-Fc fusion protein was produced and shown to bind to both monocytes and VLRB+ lymphocytes, and not to neutrophils or VLRA+ lymphocytes. The lamprey IL-17RA1 recombinant protein is directly associated with the IL-17D-Fc protein. These findings are consistent with the hypothesis that IL-17D produced by VLRA+ lymphocytes may interact with a cognate IL-17R on VLRB+ lymphocytes and monocytes to facilitate immune responses in lampreys.
Keywords:
Interleukin-17,
interleukin-17 receptor,
Lampreys,
variable lymphocyte receptors,
Adaptive Immunity
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Han
Q,
Hirano
M and
Cooper
M
(2013). Characterization of IL-17 and IL-17 receptor families in lampreys.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01037
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Received:
30 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Max Cooper, Emory University, Emory Vaccine Center and Department of Pathology and Laboratory Medicine, Atlanta, Georgia, 30322, United States, mdcoope@emory.edu