Alliin diminished pro-inflammatory cytokines in supernatants of an in vitro model of adipose tissue.
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1
Universidad de Guadalajara, Departamento de Farmacobiologia, CUCEI, Mexico
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2
Universidad de Guadalajara, Departamento de Biologia Celular y Molecular, CUCBA, Mexico
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3
Universidad de Guadalajara, Departamento de Fisiologia, CUCS, Mexico
Obesity is accompanied by chronic inflammation and is characterized as a disorder having an imbalance between pro-inflammatory and anti-inflammatory mediators. A high–fat diet can increase LPS absorption from gut in to the circulation. In another hand, Alliin a sulfoxide component of garlic has been reported as a potent anti-diabetic and anti-inflammatory. 3T3-L1 cell line has been considered for years, an in vitro model of adipose tissue (AT). We differentiated cells to mature adipocytes, we added a treatment with alliin (0.1mM) for 24 hours, after that we stimulated adipocytes with LPS (100ng/ml) for an hour. Cytokines and chemokines: IL-6, MCP-1, TNF-alfa and Adiponectin were collected after LPS stimulus and stored until quantification using ELISA techniques (Bio-Plex ProTM Assayes; Bio –Rad Laboratories, Inc.). Each sample was measured in triplicated, values were expressed as mean ± standard error of the mean (SEM). GraphPad Prism software was employed and one- way ANOVA test applied to compare the treatment effect. Significant differences were established by the Tukey-Kramer test. Values of p<0.05 were considered statistically significant. Protein levels detected after the LPS stimulus, which are significantly reduced by Alliin pre-treatment, were IL-6 and MCP-1. Moreover, we observed a reduction in TNF-alfa levels, although this was small and did not reach statistical significance. Additionally, we tested for Adiponectin levels because this represents an important union between obesity and insulin resistance and is considered an anti-inflammatory protein. We detected a large amount of Adiponectin, which is clearly reduced by LPS stimuli in the control group of adipocytes. The group pre-trated with Alliin, a slight increase can be observed in the production of this protein; however it cannot overcome the severe reduction elicited by LPS. These results provide evidences supporting Allin action, which reverses the pro-inflammatory effect of LPS in this in vitro model of adipose tissue.
Acknowledgements
Project partially supported to Lopez-Roa RI by PROII-2008A
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Keywords:
Obesity,
Inflammation,
Alliin,
Cytokines,
LPS
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Translational immunology and immune intervention
Citation:
Lopez-Roa
RI,
Quintero-Fabian
SM,
Viveros-Paredes
JM,
Garcia-Iglesias
T and
Ortuño-Sahagun
D
(2013). Alliin diminished pro-inflammatory cytokines in supernatants of an in vitro model of adipose tissue..
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01054
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Received:
30 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Rocio I Lopez-Roa, Universidad de Guadalajara, Departamento de Farmacobiologia, CUCEI, Guadalajara, Mexico, rockroa2000@yahoo.es