Comparison between enzyme-linked immuno-sorbent assay and multiplex assay for serological markers of Celiac Disease detection
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1
Hospital General Universitario Gregorio Marañón, Spain
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2
Hospital Clínico San Carlos, Spain
Objective: To compare ELISA with multiplex assay for anti-deamidated gliadin peptide (anti-DGP) IgG and anti-tissue transglutaminase (anti-tTG) IgA antibodies detection in order to identify the best according to sensivity and specificity.
Methods: 101 patients with suspicion or diagnostic of celiac disease (CD) were selected. Each serum was tested by ELISA (Orgentec Diagnostika) and multiplex assay (Bioplex 2200, Bio-Rad) for the anti-DGP IgG and anti-tTG IgA autoantibodies quantification. The association with clinical features was examinated by systematical review of the clinical histories. Also previous CD markers determinations, biopsy and HLA features were studied.
Results: Compared to the clinical, biopsy and HLA features, the ELISA showed a sensitivity of 96.15% and a specificity of 38.09 (Positive predictive value (PPV): 79.36%; Negative predictive value (NPV): 80%), whereas multiplex assay had a sensitivity of 79.59% and a specificity of 100% (PPV: 100%; NPV: 68.75%) for anti-DGP IgG antibodies detection. Furthermore, the ELISA showed a sensitivity of 68.42% and a specificity of 100% (PPV: 100%; NPV: 93.18%), and multiplex assay had a sensitivity of 100% and a specificity of 96.34% (PPV: 86.36%; NPV: 100%) for anti-tTG IgA antibodies testing.
Conclusions: Taking into consideration the previous CD markers determination and clinical, biopsy and HLA features, ELISA showed a higher sensitivity for anti-DGP IgG antibodies, but specificity was better for multiplex assay. In addition, ELISA showed a higher specificity and multiplex had a better sensitivity for anti-tTG IgA antibodies detection.
Keywords:
Celiac Disease,
ELISA,
Multiplex assay,
Anti-deamidated gliadin peptide IgG antibodies,
Anti-tissue transglutaminase IgA antibodies
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Immune-mediated disease pathogenesis
Citation:
Martín-Martín
L,
Herráiz-Nicuesa
L,
Oleo-Pérez
Y,
Sánchez-Ramón
S and
Rodriguez-Mahou
M
(2013). Comparison between enzyme-linked immuno-sorbent assay and multiplex assay for serological markers of Celiac Disease detection.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01057
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Received:
10 Jul 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Miss. Lydia Martín-Martín, Hospital General Universitario Gregorio Marañón, Madrid, Spain, lydia.martin@salud.madrid.org