Xanthine oxidase and inducible NOSynthase stress failed to efficiently suppress Leishmania major in BALB/c bone marrow dendritic cells
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1
University of Sciences and Technology Houari Boumediene, Cellular and molecular biology laboratory, Algeria
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2
Université Libre de Bruxelles, Gosselies, Belgium., Belgium
Dendritic cells play a critical role in the initiation of immune response against Leishmania parasites. Moreover, phagocytes dependent reactive oxygen species (ROS) and nitric oxide (NO) are potent leishmanicidal agents.
This study was performed with BALB/c mice bone marrow dendritic cells (BMDC) differentiated by 20 ng/ml GM-CSF for 8 days.
BMDC were infected (1/10: cell/parasite) for 6 h and 24 h with a local strain of Leishmania major LIPA35 promastigotes (MHOM/DZ/06/LIPA35, Institut Pasteur Alger). The parasite load (percentage and index of DCs infection: Parasites number/DCs) was assayed on cytospin slides stained with May Grunwald Giemsa. Nitric oxide levels were determined in cultures supernatants, using Griess reaction. The cells were collected and used for the determination of xanthine oxidase (XO) by the uric acid assay.
Our results showed that Leishmania major enhanced XO activity by 76% (P < 0.05) and 235% (P < 0.001), respectively at hours 6 and 24, compared to control. Leishmania infection increased the nitrites levels by 52% (P < 0.01) only at 24h post infection compared to control DCs. In parallel, Leishmania major infected DCs by 50.33 ±3.05 at 6h vs. 47.66± 4.16 at 24h with an index of infection 2.39 ±0.33 vs. 2.47± 0.16, respectively.
In conclusion, these results suggested that oxidative stress via increasing xanthine oxidase activity and iNOS up regulation impaired Leishmania major killing by BMDC.
Keywords:
Leishmania major,
Dendritic Cells,
Xanthine Oxidase,
iNOSynthase,
Reactives oxygen species
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Host-pathogen interactions
Citation:
Tounsi
N,
Meghari
S,
Moser
M and
Djerdjouri
B
(2013). Xanthine oxidase and inducible NOSynthase stress failed to efficiently suppress Leishmania major in BALB/c bone marrow dendritic cells.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01063
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Received:
30 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Nabila Tounsi, University of Sciences and Technology Houari Boumediene, Cellular and molecular biology laboratory, Bab Ezzouar, Algiers, 16111, Algeria, analib77@yahoo.fr
Prof. Bahia Djerdjouri, University of Sciences and Technology Houari Boumediene, Cellular and molecular biology laboratory, Bab Ezzouar, Algiers, 16111, Algeria, djerdjouri_dz@yahoo.fr