Adjuvants as possible solution to polysaccharide unconjugated vaccines
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1
Finlay Institute, Immunology Department, Cuba
Background. Plain polysaccharide (Ps) vaccines were the first meningococcal vaccines developed. Nevertheless, they neither function in new born / infant nor induce memory response and could induce antibody hyporesponsiveness. Therefore, covalent conjugated vaccines offer a particular type of stimulation where the carrier gives the T cell help. However, adjuvants in the restricted sense never were considered as an alternative solution. Therefore, we developed a proprietary adjuvant Finlay platform derived from Neisseria meningitidis. Aims. To determine if adjuvants could be use as an alternative solution to covalent conjugated Ps vaccines. Results. The Ps used were: PsVi from Salmonella Typhi and PsA from N. meningitidis serogroups A, respectively. The adjuvants used were: Outer membrane vesicles from N. meningitidis serogroups A, W and AFCo (Cochleate) 1 derived from PL. MenAfricVac™ as well as unconjugated plain Ps were use as controls. Anti Ps IgG class and IgG1, IgG2a, and IgG3 subclasses were evaluated after two parenteral or three mucosal doses. The conventional memory response was evaluated after a booster dose. Non-conventional T and B memory response was also evaluated. OMV and AFCo1 overcome the T-independence of Ps inducing a Th1 typical pattern. They induce memory response at B and T cell levels. Conclusion. Adjuvants could be an alternative to covalent conjugated vaccines. The mechanism of T cell memory induction needs to be fully explored.
Acknowledgements
We thank the Finlay Institute for supporting this work.
Keywords:
adjuvant,
Vaccine,
Protoliposome,
Cochleate,
OMV
Conference:
15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.
Presentation Type:
Abstract
Topic:
Adaptive Immunity
Citation:
Romeu
B,
Lastre
M,
Cabrera
O,
Gonzalez
E,
Mandariote
A,
García
L,
Cardoso
D and
Pérez
O
(2013). Adjuvants as possible solution to polysaccharide unconjugated vaccines.
Front. Immunol.
Conference Abstract:
15th International Congress of Immunology (ICI).
doi: 10.3389/conf.fimmu.2013.02.01074
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Received:
28 Jun 2013;
Published Online:
22 Aug 2013.
*
Correspondence:
Dr. Belkis Romeu, Finlay Institute, Immunology Department, Havana, Havana, Cuba, bromeu@finlay.edu.cu